Global DNA Methylation ELISA Kit (5’-methyl-2’-deoxycytidine Quantitation)

The Global DNA Methylation ELISA Kit is a competitive ELISA for the quantitative measurement of 5-methylcytosine (5MedCyd) . The unknown 5MedCyd samples or 5MedCyd standards are first added to a 5MedCyd DNA conjugate coated EIA plate. After a brief incubation, an anti-5MedCyd monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The 5MedCyd content in unknown samples is determined by comparison with a predetermined 5MedCyd standard curve.

Product Specifications

Overview

For detection and quantitation of 5'-methyl-2'-deoxycytidine (5MedCyd) /Suitable for use with DNA samples such as cell or tissue genomic DNA/Detection sensitivity of 150 nM 5MedCyd/

CAS Number

7732-18-5

UNSPSC

41116158

Hazard Statement

Excepted Quantities

Detection Method

Colorimetric

Features & Benefits

For detection and quantitation of 5'-methyl-2'-deoxycytidine (5MedCyd); Suitable for use with DNA samples such as cell or tissue genomic DNA; Detection sensitivity of 150 nM 5MedCyd

Shipping Conditions

2 parts: 1 RT / 1 Blue Ice

Storage Conditions

Upon receipt, aliquot and store the 5MedCyd Standard and 5MedCyd DNA Conjugate at -20ºC to avoid multiple freeze/thaw cycles. Store all other components at 4ºC.