OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence)
The OxiSelect™ In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.
Product Specifications
Overview
Measures total reactive oxygen species and reactive nitrogen species, including hydrogen peroxide, nitric oxide, peroxyl radical, and peroxynitrite anion/Suitable for use with serum, plasma, urine, cell lysates or cell culture supernatants/Detection sensitivity limit of 10 pM for DCF and 40 nM for hydrogen peroxide/
UNSPSC
41116100
Detection Method
Fluorometric
Features & Benefits
Measures total reactive oxygen species and reactive nitrogen species, including hydrogen peroxide, nitric oxide, peroxyl radical, and peroxynitrite anion; Suitable for use with serum, plasma, urine, cell lysates or cell culture supernatants; Detection sensitivity limit of 10 pM for DCF and 40 nM for hydrogen peroxide
Shipping Conditions
Room Temp
Storage Conditions
Upon receipt, store the DCF-DiOxyQ and DCF Standard at -20ºC. Avoid multiple freeze/thaw cycles. Store all other components at 4ºC.
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Classification
Oxidative Stress/ Damage->Reactive Oxygen Species (ROS) Assays->In Vitro ROS/RNS Assay
lmo Content
None
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