Total Phosphatidic Acid Assay Kit (Fluorometric)

Our Total Phosphatidic Acid Assay Kit measures total phosphatidic acid content (PA and LPA) in cell lysate samples by a coupled enzymatic reaction system. First, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. Next, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit’s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. Phosphatidic Acid levels in unknown sampled are determined based on the provided phosphatidic acid standard curve.

Product Specifications

Overview

Detects total phosphatidic acid content (PA and LPA) in cell lysate samples/Detection sensitivity of approximately 5 uM phosphatidic acid/Phosphatidic Acid standard curve included/

UNSPSC

41116100

Detection Method

Fluorometric

Features & Benefits

Detects total phosphatidic acid content (PA and LPA) in cell lysate samples; Detection sensitivity of approximately 5 uM phosphatidic acid; Phosphatidic Acid standard curve included

Shipping Conditions

Dry Ice

Storage Conditions

Store the entire kit at -80ºC. Avoid multiple freeze/thaws by aliquoting. The Fluorometric Probe is light sensitive and should be maintained in amber tubes.