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NAD+/NADH Assay Kit

Our NAD+/NADH Assay Kit detects NAD+ and NADH in cell and tissue lysates. Total NAD+/NADH can be detected or samples can be treated with an acid or base treatment to specifically detect NAD+ or NADH. This assay uses an enzymatic cycling reaction that reduces NAD+ to NADH, which then reacts with a colorimetric probe and is detected with a spectrophotometric plate reader at 450nm. NAD+/NADH levels in unknown samples are calculated based on the provided NAD+ standard curve.

Product Specifications

Overview

Detects NAD+, NADH, or total NAD+/NADH/Compatible with cell or tissue lysates/NAD+ standard included/

UNSPSC

41116100

Detection Method

Colorimetric

Features & Benefits

Detects NAD+, NADH, or total NAD+/NADH; Compatible with cell or tissue lysates; NAD+ standard included

Shipping Conditions

2 parts: 1 RT / 1 Blue Ice

Storage Conditions

Upon receipt, store the NAD Cycling Enzyme and NAD+ Standard at -80°C. Store the Colorimetric Probe at -20°C. Store the remaining components at 4°C.

Classification

Metabolism Research->Cellular Metabolism->NAD+/NADH Assay

lmo Content

None

Frequently Asked Questions

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