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Radius™ 96-Well Cell Migration Assay

The Radius™ Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius™ assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius™ gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius™ gel, creating a cell-free zone. Following cell seeding the Radius™ gel is removed, allowing migratory cells to move across the area and close the gap.

Product Specifications

Overview

Proprietary biocompatible hydrogel creates a circular area across which cells may migrate following gel removal/Versatile plate format allows use with cells of any size; no need to worry about selecting cell culture inserts with the proper pore size/Allows qualitative, quantitative, endpoint or real-time analysis/Adaptable to liquid handling equipment and HCS instrumentation

UNSPSC

41116100

Hazard Statement

Excepted Quantities

Detection Method

Light Microscopy

Features & Benefits

Proprietary biocompatible hydrogel creates a circular area across which cells may migrate following gel removal; Versatile plate format allows use with cells of any size; no need to worry about selecting cell culture inserts with the proper pore size; Allows qualitative, quantitative, endpoint or real-time analysis

Shipping Conditions

Room Temp

Storage Conditions

Upon receipt, aliquot and store the Radius™ Gel Removal Solution and DAPI Fluorescence Stain at -20ºC (avoid multiple freeze/thaw cycles), and transfer the Fixation Solution to 4ºC. All other kit components should be stored at room temperature until the kit’s expiration date.

Classification

Cell Based Assays->Cell Migration, Invasion and Wound Healing->Gap Closure Assays->Cell Migration Assays, 2D Gap Closure

lmo Content

None

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