Nuclear/Cytosolic Fractionation Kit
Cell Biolabs’ Nuclear/Cytosolic Fractionation Kit provides a simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells. The procedure has been optimized to provide extraction, with high protein recovery and low cross-contamination, in less than 2 hours. The extracted protein fractions are functional and suitable for downstream assays such as DNA footprinting, RNA splicing, gel shift assays (EMSA), reporter assays, enzyme activity assays, and Western blotting.
Product Specifications
Overview
Simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells/Extraction in less than 2 hours/Each prep can accommodate up to 5 x 106cells/
UNSPSC
41105500
Features & Benefits
Simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells; Extraction in less than 2 hours; Each prep can accommodate up to 5 x 106cells
Shipping Conditions
2 parts: 1 RT / 1 Blue Ice
Storage Conditions
Upon receipt, aliquot and store Dithiothreitol and Protease Inhibitor Cocktail at -20ºC and avoid multiple freeze/thaw cycles. Store all other components at 4ºC.
Classification
Cell Signaling and Protein Biology->Protein Analysis Tools->Protein Isolation and Extraction->Nuclear/Cytosolic Fractionation Kits
lmo Content
None
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