Nuclear/Cytosolic Fractionation Kit

Cell Biolabs’ Nuclear/Cytosolic Fractionation Kit provides a simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells. The procedure has been optimized to provide extraction, with high protein recovery and low cross-contamination, in less than 2 hours. The extracted protein fractions are functional and suitable for downstream assays such as DNA footprinting, RNA splicing, gel shift assays (EMSA), reporter assays, enzyme activity assays, and Western blotting.

Product Specifications

Overview

Simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells/Extraction in less than 2 hours/Each prep can accommodate up to 5 x 106cells/

UNSPSC

41105500

Features & Benefits

Simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells; Extraction in less than 2 hours; Each prep can accommodate up to 5 x 106cells

Shipping Conditions

Blue Ice

Storage Conditions

Upon receipt, aliquot and store Dithiothreitol and Protease Inhibitor Cocktail at -20ºC and avoid multiple freeze/thaw cycles. Store all other components at 4ºC.