Platinum-E Retroviral Packaging Cell Line, Ecotropic

Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Product Specifications

Overview

Higher retroviral yields: average titer 106 to 107 infectious units/mL with transient transfection/Longer stability: up to 4 months in the presence of drug selection/Produces ecotropic retrovirus, which can only readily infect mouse or rat cells/

UNSPSC

41106514

Features & Benefits

Higher retroviral yields: average titer 106 to 107 infectious units/mL with transient transfection; Longer stability: up to 4 months in the presence of drug selection; Produces ecotropic retrovirus, which can only readily infect mouse or rat cells

Shipping Conditions

Dry Ice

Storage Conditions

For best results begin culture upon receipt. If this is not possible, store at -80C until first culture. Store subsequent cultured cells in liquid nitrogen.