Crotonaldehyde Antibody: ATTO 488
Mouse Anti-Crotonaldehyde Monoclonal IgG1
Product Specifications
Background
Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. Crotonaldehyde (CRA) is a carcinogenic, conjugated aldehyde produced endogenously via lipid peroxidation. It covalently modifies biomolecules, forming adducts with DNA and nucleophilic amino acid residues of proteins such as lysine (1).
CAS Number
9007-83-4
Specifications
Specific for Crotonaldehyde modified proteins. Does not cross-react with Acrolein, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
Product Name Alternative
Crotonaldehyde Antibody, Croton aldehyde Antibody, Crotonaldehyde (CRA) Antibody, CRA Antibody, Crotonaldehyde conjugated protein antibody, Crotonaldehyde modified protein antibody, Crotonaldehyde-conjugated protein antibody, Crotonaldehyde-modified protein antibody, CRA-lysine adduct Antibody, Crotonaldehyde-lysine adduct Antibody
Synonyms
Crotonaldehyde Antibody, Clone 2A8.1: ATTO 488
UNSPSC
12352203
Host
Mouse
Species Reactivity
Species Independent
Immunogen
Crotonaldehyde modified Keyhole Limpet Hemocyanin (KLH)
Target
Crotonaldehyde
Clonality
Monoclonal
Isotype
IgG1
Clone
2A8.1
Conjugation
ATTO 488
Validated Applications
IHC,ICC/IF
Purification
Protein G Purified
Limit Of Detection
A 1:1000 dilution of SMC-534 was sufficient for detection of Crotonaldehyde-modified proteins in 1 µg of Crotonaldehyde-BSA conjugate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Concentration
1 mg/ml
Dilution
WB (1:1000); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
Weight
0.1
Buffer
640.91mM DMSO, 136.36mM Ethanolamine, 9.09mM Sodium Bicarbonate in 90.9% PBS
Precautions
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
References & Citations
1. Ichihashi, K. et al. (2001) J Biol Chem. 276, 23903-23913.
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