Dityrosine Antibody, Clone 10A6: HRP

Mouse Anti-Dityrosine (DT) Monoclonal IgG1

Product Specifications

Background

Dityrosine is a stable, covalent cross-link formed between tyrosine residues in proteins under oxidative stress. It is generated by reactive oxygen species (ROS) such as hydroxyl radicals and catalyzed by peroxidases and UV radiation. As a biomarker of protein oxidation, dityrosine reflects cumulative oxidative damage in tissues. In the brain, elevated dityrosine levels are associated with protein aggregation, mitochondrial dysfunction, and neuroinflammation—features common to Alzheimer’s, Parkinson’s, and other neurodegenerative diseases. Monitoring dityrosine formation provides insight into oxidative stress burden and may aid in evaluating antioxidant therapies and disease progression in neurodegenerative research.

CAS Number

9007-83-4

Specifications

Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.

Product Name Alternative

Dityrosine, Dityrosine (DT), DT, DY

Synonyms

Dityrosine Antibody, Clone 10A6: HRP

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Host

Mouse

Species Reactivity

Species Independent

Immunogen

Synthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH) .

Target

Dityrosine

Clonality

Monoclonal

Isotype

IgG1

Clone

10A6

Conjugation

HRP

Type

Monoclonal

Applications

WB | ICC/IF | FACS | FCM | ELISA

Validated Applications

WB,IHC,ELISA

Field of Research

Purification

Protein G Purified

Limit Of Detection

A 1:1000 dilution of SMC-521 was sufficient for detection of dityrosine in 1 µg of Dityrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000) ; ICC/IF (1:50) ; FACS (1:50) ; FCM (1:50) ; ELISA (1:1000) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Malencik, D. A., Anderson, S. R. (2003) Amino Acids. 25(3-4):233-247. 2. Giulivi, C., Davies, K. J. A. (2001) J Biol Chem. 276, 24129-24136.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Specificity

Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.

Background Reference 01

1. Malencik, D. A., Anderson, S. R. (2003) Amino Acids. 25 (3-4) :233-247. 2. Giulivi, C., Davies, K. J. A. (2001) J Biol Chem. 276, 24129-24136.

Species

Species Independent

Isotype

IgG1

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

MR210875A1V 250 µL

Mouse Atp6v0a4 (NM_080467) AAV Particle

Ask

View Details

ARG23941 1 Million

L1CAM Knockout Raji Polyclonal Cells

Ask

View Details

034142-ML405 100 µL

COX6A2, ID (COX6A2, COX6A, COX6AH, Cytochrome c oxidase subunit 6A2, mitochondrial, Cytochrome c oxidase polypeptide VIa-heart, Cytochrome c oxidase subunit VIA-muscle) (MaxLight 405) discontinued

Ask

View Details

RC203484L4V 200 µL

5T4 (TPBG) (NM_006670) Human Tagged ORF Clone Lentiviral Particle

Ask

View Details

40274154 3x 1.0 µg DNA

RNF182 CRISPR All-in-one AAV vector set (with saCas9)(Mouse)

Ask

View Details