Dityrosine Antibody, Clone 7D4: RPE

Mouse Anti-Dityrosine (DT) Monoclonal IgG1

Product Specifications

Background

Dityrosine is a stable, covalent cross-link formed between tyrosine residues in proteins under oxidative stress. It is generated by reactive oxygen species (ROS) such as hydroxyl radicals and catalyzed by peroxidases and UV radiation. As a biomarker of protein oxidation, dityrosine reflects cumulative oxidative damage in tissues. In the brain, elevated dityrosine levels are associated with protein aggregation, mitochondrial dysfunction, and neuroinflammation—features common to Alzheimer’s, Parkinson’s, and other neurodegenerative diseases. Monitoring dityrosine formation provides insight into oxidative stress burden and may aid in evaluating antioxidant therapies and disease progression in neurodegenerative research.

CAS Number

9007-83-4

Specifications

Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.

Product Name Alternative

Dityrosine, Dityrosine (DT), DT, DY

Synonyms

Dityrosine Antibody, Clone 7D4: RPE

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Host

Mouse

Species Reactivity

Species Independent

Immunogen

Synthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH) .

Target

Dityrosine

Clonality

Monoclonal

Isotype

IgG1

Clone

7D4

Conjugation

RPE

Type

Monoclonal

Applications

WB | ICC/IF | ELISA

Validated Applications

WB,IHC,ICC/IF

Field of Research

Purification

Protein G Purified

Limit Of Detection

A 1:1000 dilution of SMC-520 was sufficient for detection of dityrosine in 1 µg of Dityrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000) ; ICC/IF (1:50) ; ELISA (1:1000) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.46mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Malencik, D. A., Anderson, S. R. (2003) Amino Acids. 25(3-4):233-247. 2. Giulivi, C., Davies, K. J. A. (2001) J Biol Chem. 276, 24129-24136.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Specificity

Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.

Background Reference 01

1. Malencik, D. A., Anderson, S. R. (2003) Amino Acids. 25 (3-4) :233-247. 2. Giulivi, C., Davies, K. J. A. (2001) J Biol Chem. 276, 24129-24136.

Species

Species Independent

Isotype

IgG1

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