Methylglyoxal Antibody, Clone 9F11: APC

Mouse Anti-Methylglyoxal (MG) Monoclonal IgG1

Product Specifications

Background

Methylglyoxal (MG) is a reactive dicarbonyl compound formed during lipid peroxidation and glycolytic metabolism. It readily reacts with proteins, nucleic acids, and lipids to form advanced glycation end products (AGEs), which accumulate in the brain during aging and neurodegeneration. These AGEs disrupt cellular homeostasis, impair mitochondrial function, and trigger inflammatory responses. MG-induced DNA crosslinking and protein modification contribute to neuronal dysfunction and apoptosis. Elevated MG levels have been detected in Alzheimer’s disease, Parkinson’s disease, and diabetic neuropathy, linking metabolic stress to neurodegenerative pathology. MG also interferes with synaptic signaling and plasticity, further compromising cognitive function. Quantification of MG and AGEs in cerebrospinal fluid and plasma serves as a diagnostic tool and a measure of oxidative burden. Targeting MG detoxification pathways, such as the glyoxalase system, holds therapeutic potential for mitigating oxidative stress and preserving neuronal integrity in neurodegenerative diseases.

CAS Number

9007-83-4

Specifications

Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, Malondialdeyhde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Crotonaldehyde modified proteins.

Product Name Alternative

Methylglyoxal, 2-Oxopropanal, 2 oxo propanal, MG, Pyruvaldehyde, Methylglyoxal (MG), MG-modified protein

Synonyms

Methylglyoxal Antibody, Clone 9F11: APC

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Host

Mouse

Species Reactivity

Species Independent

Immunogen

Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH) .

Target

Methylglyoxal

Clonality

Monoclonal

Isotype

IgG1

Clone

9F11

Conjugation

APC

Type

Monoclonal

Applications

WB | ICC/IF | FACS | FCM | ELISA

Validated Applications

IHC,ICC/IF

Field of Research

Cancer | Oxidative Stress | Lipid peroxidation

Purification

Protein G Purified

Limit Of Detection

A 1:1000 dilution of SMC-517 was sufficient for detection of Methylglyoxal in 0.5 µg of Methylglyoxal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000) ; ICC/IF (1:50) ; FACS (1:50) ; FCM (1:50) ; ELISA (1:1000) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.46mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Negre-Salvayre et al. (2008) Br J Pharmacol. 153(1):6-20. 2. Murata-Kamiya, N., Kamiya, H. (2001) Nucleic Acids Res. 29(16): 3433-3438.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Specificity

Background Reference 01

1. Negre-Salvayre et al. (2008) Br J Pharmacol. 153 (1) :6-20. 2. Murata-Kamiya, N., Kamiya, H. (2001) Nucleic Acids Res. 29 (16) : 3433-3438.

Species

Species Independent

Isotype

IgG1

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