Methylglyoxal Antibody, Clone 9E7: APC

Mouse Anti-Methylglyoxal (MG) Monoclonal IgG2a

Product Specifications

Background

Methylglyoxal (MG) is a highly reactive alpha-oxoaldehyde generated as a byproduct of glycolysis and lipid peroxidation. In the nervous system, MG contributes to oxidative stress by forming advanced glycation end products (AGEs) through reactions with amino acids and protein residues. These AGEs disrupt protein function, promote inflammation, and impair cellular signaling—hallmarks of neurodegenerative diseases. MG also crosslinks DNA polymerase and substrate DNA, inhibiting DNA replication and repair. This genotoxicity exacerbates neuronal damage and accelerates disease progression in conditions such as Alzheimer’s and Parkinson’s disease. Elevated MG levels have been observed in aging brains and in models of metabolic dysfunction, linking it to both neurodegeneration and systemic disease. Therapeutic strategies aimed at reducing MG accumulation or enhancing its detoxification via the glyoxalase system are under investigation. As a biomarker and mediator of oxidative damage, MG represents a critical target in the study and treatment of neurodegenerative disorders.

CAS Number

9007-83-4

Specifications

Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, Malondialdeyhde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Crotonaldehyde modified proteins.

Product Name Alternative

Methylglyoxal, 2-Oxopropanal, 2 oxo propanal, MG, Pyruvaldehyde, Methylglyoxal (MG), MG-modified protein

Synonyms

Methylglyoxal Antibody, Clone 9E7: APC

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Host

Mouse

Species Reactivity

Species Independent

Immunogen

Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH) .

Target

Methylglyoxal

Clonality

Monoclonal

Isotype

IgG2a

Clone

9E7

Conjugation

APC

Type

Monoclonal

Applications

WB | ICC/IF | IHC | FACS | FCM | ELISA

Validated Applications

IHC,ICC/IF

Field of Research

Cancer | Oxidative Stress | Lipid peroxidation

Purification

Protein G Purified

Limit Of Detection

A 1:1000 dilution of SMC-516 was sufficient for detection of Methylglyoxal in 0.5 µg of Methylglyoxal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000) ; ICC/IF (1:50) ; FACS (1:50) ; FCM (1:50) ; ELISA (1:1000) ; IHC (1:50) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.46mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Negre-Salvayre et al. (2008) Br J Pharmacol. 153(1):6-20. 2. Murata-Kamiya, N., Kamiya, H. (2001) Nucleic Acids Res. 29(16): 3433-3438.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Specificity

Background Reference 01

1. Negre-Salvayre et al. (2008) Br J Pharmacol. 153 (1) :6-20. 2. Murata-Kamiya, N., Kamiya, H. (2001) Nucleic Acids Res. 29 (16) : 3433-3438.

Species

Species Independent

Isotype

IgG2a

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