Malondialdehyde Antibody, Clone 11E3: RPE

Malondialdehyde (MDA) is a highly reactive, low-molecular-weight aldehyde and one of the most widely used biomarkers of oxidative stress due to its chemical stability and ease of detection. Formed as a byproduct of lipid peroxidation, MDA reflects oxidative damage to polyunsaturated fatty acids in cellular membranes—a process central to the pathology of neurodegenerative diseases. MDA exhibits a strong affinity for amino groups, forming adducts with free amino acids, proteins, and nucleic acids. These adducts can disrupt protein function and induce DNA cross-linking, contributing to mutagenesis and cellular dysfunction. Elevated MDA levels have been consistently observed in Alzheimer’s disease and other neurodegenerative conditions, as well as in systemic diseases such as diabetes and cancer. In neuroscience research, MDA serves not only as a diagnostic and prognostic marker but also as a mechanistic link between oxidative stress and neuronal injury. Its quantification—commonly performed using the thiobarbituric acid reactive substances (TBARS) assay—provides valuable insights into the redox state of the brain and the efficacy of antioxidant therapies. Given its dual role as both a biomarker and a mediator of oxidative damage, MDA is a critical target in neurodegenerative disease research. Understanding and mitigating MDA-associated toxicity may offer new avenues for therapeutic intervention in oxidative stress-driven neurological disorders.

Specific for Malondialdehyde conjugated proteins. Does not detect free Malondialdehyde. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Methylglyoxal modified proteins.

A 1:1000 dilution of SMC-515 was sufficient for detection of Malondialdehyde in 2 µg of Malondialdehyde conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Specific for Malondialdehyde conjugated proteins. Does not detect free Malondialdehyde. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Methylglyoxal modified proteins.