Hexanoyl-Lysine adduct Antibody, Clone 5D9: ATTO 488

Mouse Anti-Hexanoyl-Lysine adduct (HEL) Monoclonal IgG1

Product Specifications

Background

Hexanoyl-lysine adduct (HEL) is a lipid peroxidation marker formed by the reaction of oxidized fatty acids with lysine residues on proteins. It serves as an early indicator of oxidative stress, a central feature in the pathogenesis of neurodegenerative diseases. In the brain, HEL accumulation reflects damage to neuronal membranes and proteins caused by reactive oxygen species (ROS) . Elevated levels of HEL have been detected in models of Alzheimerâ€™s disease, Parkinsonâ€™s disease, and other neurodegenerative conditions, correlating with disease progression and cognitive decline. HEL is particularly valuable as a biomarker due to its stability and specificity for lipid peroxidation. Its presence indicates oxidative damage at an early stage, often preceding overt neurodegeneration. This makes HEL a promising candidate for early diagnosis, disease monitoring, and evaluating the efficacy of antioxidant therapies. By highlighting the oxidative component of neurodegeneration, HEL contributes to a deeper understanding of disease mechanisms and supports the development of targeted interventions aimed at reducing oxidative injury in the nervous system.

CAS Number

9007-83-4

Specifications

Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine. Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.

Product Name Alternative

Hexanoyl-Lysine adduct, HEL (Hexanoyl-Lysine adduct), HEL, HEL Adduct, Hexanoyl-Lys adduct, Hexanoyl-Lys, Hexanoyl-Lysine (HEL) adduct, Hexanoyl-Lys (HEL), Hexanoyl Lysine adduct, Hexanoyl-Lysine adduct-modified protein, Hexanoyl-Lysine Adduct (HEL)

Synonyms

Hexanoyl-Lysine adduct Antibody, Clone 5D9: ATTO 488

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Host

Mouse

Species Reactivity

Species Independent

Immunogen

Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH) .

Target

Hexanoyl-Lysine adduct

Clonality

Monoclonal

Isotype

IgG1

Clone

5D9

Conjugation

ATTO 488

Type

Monoclonal

Applications

WB | ICC/IF | FACS | FCM | ELISA | IHC

Validated Applications

IHC,ICC/IF

Field of Research

Cancer | Oxidative Stress | Lipid peroxidation

Purification

Protein G Purified

Limit Of Detection

A 1:1000 dilution of SMC-508 was sufficient for detection of Hexanoyl Lysine adduct in 0.5 µg of Hexanoyl Lysine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000) ; ICC/IF (1:50) ; ELISA (1:1000) ; FACS (1:50) ; FCM (1:50) ; IHC (1:100) ; optimal dilutions for assays should be determined by the user.