Acrolein Antibody, Clone 2H2: ATTO 488

Lipid peroxidation, a hallmark of oxidative stress, occurs when reactive oxygen species (ROS) attack unsaturated lipids in cellular membranes. This process not only compromises membrane integrity but also generates toxic byproducts that disrupt cellular homeostasis. Among these, acrolein—a highly reactive α, β-unsaturated aldehyde—has emerged as a critical factor in the pathogenesis of neurodegenerative diseases. Acrolein is a terminal product of lipid peroxidation and is significantly more reactive than other aldehydes. It forms stable adducts with nucleophilic sites in DNA and proteins, particularly targeting cysteine, histidine, and lysine residues. These modifications impair protein function, alter signaling pathways, and contribute to mitochondrial dysfunction and neuronal death. Elevated levels of acrolein have been consistently observed in the brains and cerebrospinal fluid of patients with Alzheimer’s disease, Parkinson’s disease, multiple sclerosis, and other neurodegenerative conditions. Its presence correlates with markers of inflammation, synaptic loss, and cognitive decline, positioning acrolein as both a biomarker of disease progression and a potential therapeutic target. In neuroscience research, acrolein is increasingly recognized for its role in amplifying oxidative damage and neuroinflammation. Understanding its molecular interactions and downstream effects is essential for developing antioxidant-based therapies and early diagnostic tools aimed at mitigating neurodegeneration.

Specific for Acrolein modified proteins. Does not detect free acrolein. Does not cross-react with Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.

Acraldehyde, Acrylaldehyde, Acrylic aldehyde, Allyl aldehyde, Propenal, 2-Propenal, Prop-2-enal, Prop-2-en-1-al, Acroleine, Paracetaldehyde, Acquinite, Aqualin, Aqualine, Biocide

A 1:1000 dilution of SMC-504 was sufficient for detection of Acrolein in 2 µg of Acrolein conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Specific for Acrolein modified proteins. Does not detect free acrolein. Does not cross-react with Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.