GABAA Receptor Alpha 5 Antibody, Clone N415/24

Mouse Anti-Human GABAA Receptor Alpha 5 Monoclonal IgG1

Product Specifications

Background

The GABA-A receptor alpha 5 subunit (GABRA5) is a critical component of the GABA-A receptor complex, the brain’s primary fast-acting inhibitory neurotransmitter system. As part of the ligand-gated ion channel superfamily, GABRA5 is predominantly expressed in the hippocampus, where it plays a central role in modulating tonic inhibition, synaptic plasticity, and cognitive function. Recent research has highlighted GABRA5 as a promising target in neurodegenerative disease, particularly Alzheimer’s disease. Inverse agonists selective for GABRA5-containing receptors have shown potential in enhancing learning and memory by reducing excessive inhibitory tone, which is often elevated in neurodegenerative states. This modulation of GABRA5 activity may help restore excitatory-inhibitory balance, improve cognitive performance, and slow disease progression. Beyond Alzheimer’s, GABRA5 is also being investigated for its role in age-related cognitive decline and other neurological disorders characterized by impaired GABAergic signaling. Its unique pharmacological profile and region-specific expression make it an attractive candidate for precision therapies aimed at cognitive enhancement and neuroprotection. As neuroscience advances toward targeted interventions, GABRA5 stands out as a key molecular player in the development of next-generation treatments for neurodegenerative diseases.

CAS Number

9007-83-4

Specifications

Detects ~55kDa. Does not cross-react with GABA A Receptor Alpha2 or other GABA-A Receptors based on KO validation data.

Product Name Alternative

Gamma-aminobutyric acid receptor subunit alpha-5, GABA (A) receptor subunit alpha-5, GABRA5, Gamma aminobutyric acid A receptor alpha 5, GBRA5_HUMAN

Synonyms

GABA A Receptor Alpha 5 Antibody, Clone N415/24

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

2558

Swiss Prot

P31644

Accession Number

NP_000801.1

Cellular Locus

Cell Junction | Synapse | Postsynaptic Cell Membrane | Cell Membrane

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein amino acids 368-419 (last cytoplasmic domain before extracellular C-terminus) of human GABA A Receptor Alpha5.

Target

GABA A Receptor

Clonality

Monoclonal

Isotype

IgG1

Clone

N415/24 (Formerly sold as S415-24)

Conjugation

Unconjugated

Type

Monoclonal

Applications

WB | IHC | ICC/IF

Validated Applications

WB,IHC,ICC/IF

Field of Research

Purification

Protein G Purified

Limit Of Detection

A 1:100 dilution of SMC-494 was sufficient for detection of GABA-A R, Alpha5 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

PBS pH7.4, 50% glycerol, 0.1% sodium azide *Storage buffer changes when conjugated

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136. 2. Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602. 3. Hengen K.B., et al. (2012) PLoS One. 7(1): e30608.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

-20ºC

Specificity

Detects ~55kDa. Does not cross-react with GABA A Receptor Alpha2 or other GABA-A Receptors based on KO validation data.

Background Reference 01

1. Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136. 2. Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602. 3. Hengen K.B., et al. (2012) PLoS One. 7 (1) : e30608.