Kir6.1 Antibody, Clone N366/60

Mouse Anti-Rat Kir6.1 Monoclonal IgG2A

Product Specifications

Background

Kir6.1, encoded by the KCNJ8 gene, is a member of the inwardly rectifying potassium (Kir) channel family, which plays a critical role in regulating membrane potential and cellular excitability. Unlike voltage-gated potassium channels, Kir channels preferentially allow potassium ions to flow into the cell, stabilizing the resting membrane potential and modulating neuronal responsiveness. Kir6.1 is a key subunit of ATP-sensitive potassium (K_ATP) channels, which couple cellular metabolic state to electrical activity. While Kir6.1 is predominantly expressed in cardiac and smooth muscle tissues—including fetal and adult hearts—it is also present in the brain, where it contributes to neurovascular coupling and neuronal protection under metabolic stress. In the nervous system, Kir6.1-containing K_ATP channels are activated during conditions of energy depletion, such as ischemia or oxidative stress, helping to hyperpolarize neurons and reduce excitotoxicity. Dysregulation of Kir6.1 function has been implicated in neurodegenerative diseases, including stroke, Alzheimer’s disease, and Parkinson’s disease, where impaired energy metabolism and ionic imbalance are central pathological features. Furthermore, mutations in KCNJ8 have been associated with J-wave syndromes and other channelopathies, highlighting the broader physiological importance of Kir6.1 in excitable tissues. As research into metabolic regulation and ion channel dysfunction in neurodegeneration advances, Kir6.1 is emerging as a promising target for therapeutic intervention aimed at preserving neuronal function and preventing cell death.

CAS Number

9007-83-4

Specifications

Detects ~45kDa.

Product Name Alternative

Kir6.1, KCNJ8, Kcnj8, Inward rectifier K (+) channel Kir6.1, Potassium channel inwardly rectifying subfamily J member 8, ATP-sensitive inward rectifier potassium channel 8, uKATP-1

Synonyms

Kir6.1 Antibody, Clone N366/60

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

25472

Swiss Prot

Q63664

Accession Number

NP_058795.3

Cellular Locus

Membrane

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein amino acids 306-424 (Cytoplasmic C-terminus) of rat Kir6.1

Target

Kir6.1

Clonality

Monoclonal

Isotype

IgG2A

Clone

N366/60 (Formerly sold as S366-60)

Conjugation

Unconjugated

Type

Monoclonal

Applications

WB | IHC | ICC/IF

Validated Applications

WB,IHC,ICC/IF

Field of Research

Neuroscience | Ion channels | Potassium Channels | Inward-Rectifying Potassium Channels | Cancer

Purification

Protein G Purified

Limit Of Detection

A 1:100 dilution of SMC-491 was sufficient for detection of Kir6.1 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

PBS pH7.4, 50% glycerol, 0.1% sodium azide *Storage buffer changes when conjugated

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Zobel C., et al. (2003) J Physiol. 550: 365-372. 2. Panama B.K., McLerie M., and Lopatin A.N. (2007) Am J Physiol Heart Circ Physiol. 293: H3558-H3567. 3. Munoz V., et al. (2007) Heart Rhythm. 4(4): 487-496. 4. Aguilar-Bryan L., et al. (1998) Physiol Rev. 78(1): 227-245.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

-20ºC

Specificity

Detects ~45kDa.

Background Reference 01

1. Zobel C., et al. (2003) J Physiol. 550: 365-372. 2. Panama B.K., McLerie M., and Lopatin A.N. (2007) Am J Physiol Heart Circ Physiol. 293: H3558-H3567. 3. Munoz V., et al. (2007) Heart Rhythm. 4 (4) : 487-496. 4. Aguilar-Bryan L., et al. (1998) Physiol Rev. 78 (1) : 227-245.