Nav beta 3 Antibody, Clone N396/29

Mouse Anti-Rat Nav beta 3 Sodium Channel Monoclonal IgG2B

Product Specifications

Background

NaVβ3 (encoded by SCN3B) is a regulatory subunit of voltage-gated sodium channels that modulates channel gating kinetics and neuronal excitability. Unlike other β subunits, NaVβ3 slows inactivation and promotes persistent sodium currents, which enhance synaptic input summation and increase neuronal responsiveness. This property may contribute to hyperexcitability in specific neuronal populations, a feature observed in epilepsy and neurodegenerative disorders. NaVβ3 also interacts with neurofascin, facilitating the localization and retention of sodium channels at the nodes of Ranvier in developing and mature myelinated axons. This interaction is essential for saltatory conduction and efficient neural signaling. Disruption of SCN3B has been linked to Brugada syndrome, highlighting its role in excitable tissue function. In the context of neurodegeneration, altered NaVβ3 expression or function may contribute to impaired action potential propagation, synaptic dysfunction, and neuronal vulnerability. Its role in modulating persistent sodium currents suggests a potential link to excitotoxicity and calcium overload, both of which are implicated in diseases such as ALS and Alzheimer’s disease. NaVβ3 thus represents a promising target for understanding and modulating neuronal excitability in neurodegenerative conditions.

CAS Number

9007-83-4

Specifications

Detects ~40kDa. Does not cross-reat with NavBeta1, Navbeta2, or Navbeta4.

Product Name Alternative

NaVbeta3, SCN3B, Scn3b, Sodium channel subunit beta-3, KIAA1158

Synonyms

NaVbeta3 Antibody, Clone N396/29

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

245956

Swiss Prot

Q9JK00

Accession Number

NP_620797.1

Cellular Locus

Membrane

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein amino acids 1-215 (full-length) of rat NavBeta3

Target

NaVbeta3

Clonality

Monoclonal

Isotype

IgG2B

Clone

N396/29 (Formerly sold as S396-29)

Conjugation

Unconjugated

Type

Monoclonal

Applications

WB | ICC/IF

Validated Applications

WB,ICC/IF

Field of Research

Neuroscience | Ion Channels | Sodium Channels | Voltage-Gated Sodium Channels

Purification

Protein G Purified

Limit Of Detection

A 1:100 dilution of SMC-490 was sufficient for detection of NavBeta3 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

PBS pH7.4, 50% glycerol, 0.1% sodium azide *Storage buffer changes when conjugated

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Genes and mapped phenotypes. (n.d.). Retrieved March 26, 2015, from http://www.ncbi.nlm.nih.gov/gene/?term=Q9jK00 2. Qu Y., et al. (2001) Mol Cell Neurosci. 18(5): 570-580. 3. Ishikawa T., et al. (2013) Circ J. 77(4): 956-967.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

-20ºC

Specificity

Detects ~40kDa. Does not cross-reat with NavBeta1, Navbeta2, or Navbeta4.

Background Reference 01

1. Genes and mapped phenotypes. (n.d.) . Retrieved March 26, 2015, from http://www.ncbi.nlm.nih.gov/gene/?term=Q9jK00 2. Qu Y., et al. (2001) Mol Cell Neurosci. 18 (5) : 570-580. 3. Ishikawa T., et al. (2013) Circ J. 77 (4) : 956-967.