GABAA Receptor Alpha 4 Antibody, Clone N398A/34: RPE

Mouse Anti-Rat GABA-A Receptor Alpha4 Monoclonal IgG1

Product Specifications

Background

The GABA-A receptor alpha 4 subunit (GABRA4) is a key component of the GABA-A receptor complex, the primary mediator of fast inhibitory neurotransmission in the central nervous system. As part of the ligand-gated ion channel superfamily, GABRA4 contributes to tonic inhibition, particularly in the thalamus and hippocampus—regions essential for cognition, memory, and seizure regulation. Dysregulation of GABRA4 has been strongly linked to epilepsy and pharmacoresistance to antiepileptic drugs, highlighting its role in maintaining neural circuit stability. Beyond epilepsy, emerging evidence suggests that GABRA4 dysfunction may contribute to the pathophysiology of neurodegenerative diseases by disrupting inhibitory-excitatory balance, promoting network hyperexcitability, and impairing synaptic plasticity. These mechanisms are increasingly recognized in conditions such as Alzheimer’s disease, where altered GABAergic signaling is associated with cognitive decline and neuronal vulnerability. As a result, GABRA4 is gaining attention as a potential biomarker and therapeutic target in neurodegeneration. Advancing our understanding of GABRA4’s role in inhibitory signaling may unlock new strategies for early intervention and disease modification in disorders characterized by GABAergic dysfunction.

CAS Number

9007-83-4

Specifications

Detects ~60kDa. Does not cross-react with other GABA-A-Receptor Alpha subunits.

Product Name Alternative

GABA A receptor subunit alpha 4, GABRA4, Gamma aminobutyric acid A receptor Alpha 4, Gamma-aminobutyric acid receptor subunit alpha-4, GBRA4_HUMAN

Synonyms

GABA-A Receptor Alpha 4 Antibody, Clone N398A/34: RPE

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

140675

Swiss Prot

P28471

Accession Number

NP_542154.3

Cellular Locus

Cell Junction | Synapse | Postsynaptic Cell Membrane | Cell Membrane

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein amino acids 410-450 (Cytoplasmic C-terminus) of rat GABA-A-Receptor Alpha 4

Target

GABA A Receptor

Clonality

Monoclonal

Isotype

IgG1

Clone

N398A/34 (Formerly sold as S398A-34)

Conjugation

RPE

Type

Monoclonal

Applications

WB | IHC | ICC/IF

Validated Applications

WB,IHC,ICC/IF

Field of Research

Neuroscience | Neurotransmitter Receptors | GABA Receptors | GABAA Receptors

Purification

Protein G Purified

Limit Of Detection

A 1:100 dilution of SMC-489 was sufficient for detection of GABA-A R, Alpha4 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.46mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136. 2. Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602. 3. Grabenstatter H.L., et al. (2014) Epilepsia. 55(11): 1826-1833. 4. Bethmann K., Fritschy J.M., Brandt C., Loscher W. (2008) Neurbiol Dis. 31(2): 169-187.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Specificity

Detects ~60kDa. Does not cross-react with other GABA-A-Receptor Alpha subunits.

Background Reference 01

1. Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136. 2. Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602. 3. Grabenstatter H.L., et al. (2014) Epilepsia. 55 (11) : 1826-1833. 4. Bethmann K., Fritschy J.M., Brandt C., Loscher W. (2008) Neurbiol Dis. 31 (2) : 169-187.