Ankyrin R Antibody, Clone N388A/10

Mouse Anti-Human Ankyrin R Monoclonal IgG2B

Product Specifications

Background

Ankyrin-R (ANK1), originally identified in erythrocytes, is a member of the ankyrin family of adaptor proteins that tether integral membrane proteins to the spectrin-actin cytoskeleton. This structural linkage is essential for maintaining plasma membrane stability and for the precise localization of ion channels, exchangers, and transporters across various cell types. Although Ankyrin-R is best known for its role in red blood cells—where mutations cause hereditary spherocytosis—it is also expressed in the brain and muscle tissue. In neurons, Ankyrin-R contributes to the organization of membrane domains and supports the structural integrity of axons and dendrites. Its ability to bind β-spectrin and multiple membrane proteins suggests a broader role in maintaining neuronal polarity and excitability. Recent studies have linked ANK1 dysregulation to Alzheimer’s disease, where altered expression patterns have been observed in affected brain regions. Epigenetic modifications of the ANK1 gene have been associated with neuroinflammation and neuronal vulnerability, positioning Ankyrin-R as a potential biomarker and contributor to disease progression. As interest grows in the cytoskeletal underpinnings of neurodegeneration, Ankyrin-R is gaining recognition for its role in maintaining neuronal architecture and function. Understanding its molecular interactions and regulatory mechanisms may offer new insights into the pathogenesis of Alzheimer’s and related disorders.

CAS Number

9007-83-4

Specifications

Detects ~200kDa. Does not cross-react with Ankyrin-B or Ankyrin-G.

Product Name Alternative

Ankyrin R, Ankyrin-R, Ankyrin-1, Ankyrin 1, ANK1, ANK-1, Ankyrin, Erythrocyte ankyrin, rCG_43073

Synonyms

Ankyrin R Antibody, Clone N388A/10

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

286

Swiss Prot

P16157

Accession Number

NP_000028.3

Cellular Locus

Cytoplasm | Cytoskeleton | Myofibril | Sarcomere | M line

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein amino acids 1-1881 (full-length) of human Ankyrin-R

Target

Ankyrin R

Clonality

Monoclonal

Isotype

IgG2B

Clone

N388A/10 (Formerly sold as S388A-10)

Conjugation

Unconjugated

Type

Monoclonal

Applications

WB | IHC | ICC/IF

Validated Applications

WB,IHC,ICC/IF

Field of Research

Purification

Protein G Purified

Limit Of Detection

A 1:100 dilution of SMC-487 was sufficient for detection of Ankyrin R in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000) ; optimal dilutions for assays should be determined by the user.

Weight

0.012

Buffer

PBS pH7.4, 50% glycerol, 0.1% sodium azide

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Bennett V., Baines A.J. (2001) Physiol. Rev. 81 (3): 1353–92. 2. Genes and mapped phenotypes. (n.d.). Retrieved April 21, 2015, from http://www.ncbi.nlm.nih.gov/gene/286

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

-20ºC

Specificity

Detects ~200kDa. Does not cross-react with Ankyrin-B or Ankyrin-G.

Background Reference 01

1. Bennett V., Baines A.J. (2001) Physiol. Rev. 81 (3) : 1353–92. 2. Genes and mapped phenotypes. (n.d.) . Retrieved April 21, 2015, from http://www.ncbi.nlm.nih.gov/gene/286