TCP1-alpha Antibody, Clone 91a: RPE

T-complex protein 1 subunit alpha (TCP1α) is a core component of the eukaryotic chaperonin-containing TCP1 complex (CCT or TRiC), a cytosolic molecular chaperone essential for the ATP-dependent folding of key cytoskeletal proteins such as actin and tubulin. TCP1α is ubiquitously expressed and highly conserved, playing a central role in maintaining proteostasis under both physiological and stress conditions. As part of the CCT complex, TCP1α assists in the proper folding of a wide range of client proteins involved in cell structure, intracellular transport, and signal transduction. Like heat shock proteins (e.g., HSP70), TCP1α is upregulated in response to cellular stress and exhibits ATPase activity, underscoring its role in protein quality control. In the context of neurodegenerative disease, TCP1α has gained attention for its involvement in mitigating protein misfolding and aggregation—hallmarks of disorders such as Alzheimer’s, Parkinson’s, and Huntington’s disease. Impaired chaperonin function can lead to the accumulation of toxic protein species, disrupted cytoskeletal dynamics, and neuronal dysfunction. Moreover, TCP1α may influence the degradation of misfolded proteins via the ubiquitin-proteasome system and autophagy pathways. Given its critical role in neuronal proteostasis and cytoskeletal integrity, TCP1α is emerging as a potential therapeutic target for modulating protein folding and preventing neurodegeneration.

Detects ~60kDa. Also detects ~92kDa. Cross reactivity with human HSP60 has been observed with this antibody in immunoblot analysis. Reacts weakly with Saccharomyces cerevisiae, consistent with the epitope sequence being AKLRS (instead of AKLRA). In C. elegans, it reacts with TCP1 alpha and another CCT subunit. In plants, it recognizes TCP1 of Pisum sativum, and the sequence of Arabidopsis thalania TCP1 over the region of the epitope AKLRA. It has also been shown that it reacts with a subunit of a specialized chaperonin which folds phytochrome.

1 µg/ml of SMC-479 was sufficient for detection of TCP1 alpha in 20 µg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.

Detects ~60kDa. Also detects ~92kDa. Cross reactivity with human HSP60 has been observed with this antibody in immunoblot analysis. Reacts weakly with Saccharomyces cerevisiae, consistent with the epitope sequence being AKLRS (instead of AKLRA) . In C. elegans, it reacts with TCP1 alpha and another CCT subunit. In plants, it recognizes TCP1 of Pisum sativum, and the sequence of Arabidopsis thalania TCP1 over the region of the epitope AKLRA. It has also been shown that it reacts with a subunit of a specialized chaperonin which folds phytochrome.

Human | Mouse | Rat | Bovine | Rabbit | Guinea Pig (Cavia porcellus) | Hamster | Dog | Pig | Yeast | Yeast (Saccharomyces cerevisiae) | Nematode (Caenorhabditis elegans) | Fruit Fly (Drosophila melanogaster) | Monkey