LGI1 Antibody, Clone N283/7

Mouse Anti-Mouse LGI1 Monoclonal IgG2a

Product Specifications

Background

LGI1 (Leucine-Rich Glioma Inactivated 1) is a secreted synaptic protein that modulates neuronal excitability and synaptic transmission. It interacts with ADAM22 and ADAM23 to stabilize AMPA receptor function and maintain synaptic integrity. Mutations in LGI1 are linked to autosomal dominant lateral temporal lobe epilepsy (ADTLE) and have been implicated in autoimmune encephalitis. In neurodegenerative disease research, LGI1 is gaining attention for its role in synaptic maintenance, neuroinflammation, and neuronal survival. Dysregulation of LGI1 may contribute to cognitive decline and seizure susceptibility. Its dual role in epilepsy and neurodegeneration highlights LGI1 as a valuable biomarker and therapeutic target in synaptic pathologies.

CAS Number

9007-83-4

Specifications

Detects ~60kDa.

Product Name Alternative

LGI1, Leucine-rich glioma-inactivated protein 1, Epitempin 1, EPT, ETL1, ADLTE, ADPEAF, ADPAEF, IB1099, OTTHUMP00000020121, OTTHUMP00000020122

Synonyms

LGI1 Antibody, Clone N283/7

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

56839

Swiss Prot

Q9JIA1.1

Accession Number

NP_064674.1

Cellular Locus

Cell Junction | Golgi Apparatus | Endoplasmic Reticulum

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse LGI1. Rat: 100% identity (77/77 amino acids identical) . Human: 98% identity (76/77 amino acids identical) . ~50% identity with LGI2, LGI3 and LGI4.

Target

LGI1

Clonality

Monoclonal

Isotype

IgG2a

Clone

N283/7 (Formerly sold as S283-7)

Conjugation

Unconjugated

Type

Monoclonal

Applications

WB | IHC | ICC/IF

Validated Applications

WB,IHC,ICC/IF

Field of Research

Neuroscience | Neurodegeneration | Cell Signaling | Epigenetics and Nuclear Signaling | Cancer

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-461 was sufficient for detection of LGI1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000) ; optimal dilutions for assays should be determined by the user.

Weight

0.012

Buffer

PBS pH7.4, 50% glycerol, 0.1% sodium azide

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Chernova O.B., Somerville R.P. and Cowell J.K. (1998) Oncogene. 17:2873-81. 2. Fialka F., et al. (2008) Oral Oncol. 3. Gu W., et al. (2005) Mol. Biol. Evol. 22:2209-16. 4. Fukata Y., et al. (2006) Science. 313:1792-5.