PINK1 Antibody, Clone N4/15

PINK1 (PTEN-induced putative kinase 1) is a mitochondrial serine/threonine kinase that safeguards mitochondrial integrity and function. It plays a central role in mitophagy—the selective autophagic clearance of damaged mitochondria—by phosphorylating mitochondrial substrates and recruiting the cytosolic E3 ubiquitin ligase Parkin to the outer mitochondrial membrane (OMM) . PINK1 is synthesized as a 63 kDa precursor and undergoes proteolytic processing to generate cleaved forms that localize to various sub-mitochondrial compartments. Its kinase domain faces the cytosol, enabling interactions with cytosolic proteins involved in mitochondrial quality control. Mutations in PINK1 are linked to autosomal recessive early-onset Parkinson’s disease and are associated with mitochondrial dysfunction, alpha-synuclein aggregation, and impaired proteasome activity. Loss of PINK1 function disrupts mitochondrial dynamics and neuronal survival, making it a key target in neurodegenerative disease research.

Parkinson disease (autosomal recessive) 6, EC 2.7.11.1, PARK6, serine/threonine-protein kinase PINK1 mitochondrial, PTEN-induced putative kinase protein 1, protein kinase BRPK, BRPK, FLJ27236, PTEN induced putative kinase 1, Phosphatase and Tensin Homolog

Neuroscience | Cell Signaling | Post-translational Modifications | Phosphorylation | Cardiovascular System | Heart | Neurodegeneration | Parkinson's Disease

1 µg/ml of SMC-450 was sufficient for detection of PINK1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.