LAR Antibody, Clone N165/38: RPE

Mouse Anti-Human LAR (PTPRF) Monoclonal IgG2a

Product Specifications

Background

LAR (Leukocyte Common Antigen-Related Protein), encoded by PTPRF, is a receptor-type protein tyrosine phosphatase involved in neural development and regeneration. It regulates cell adhesion, cytoskeletal dynamics, and synaptic plasticity by dephosphorylating β-catenin and modulating cadherin-mediated junctions. LAR influences neurite outgrowth, axon guidance, and actin remodeling, making it critical for neurodevelopment and repair. In neurodegenerative contexts, LAR dysregulation may impair synaptic stability and neuronal connectivity. Its role in insulin signaling and inflammation further links it to metabolic and neurodegenerative disorders. As a modulator of synaptic architecture and signaling, LAR represents a potential target for therapeutic strategies aimed at restoring neural function.

CAS Number

9007-83-4

Specifications

Detects ~85kDa (full length protein is 210 kDa – smaller due to proteolysis into P-subunit containing transmembrane and intracellular domains.

Product Name Alternative

PTPRF, LAR, Leukocyte antigen related, Leukocyte antigen related PTP receptor, Leukocyte antigen related tyrosine phosphatase, Leukocyte common antigen related, Receptor-type tyrosine-protein phosphatase F, Receptor type tyrosine protein phosphatase F precursor, Protein Tyrosine Phosphatase Receptor Type F, Protein tyrosine phosphatase receptor type F polypeptide, Receptor linked phosphatase LAR, LAR protein, LARS, LARFN5C, LCA homolog, FLJ43335, FLJ45062, FLJ45567

Synonyms

LAR/PTPRF Antibody, Clone N165/38: RPE

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

5792

Swiss Prot

P10586

Accession Number

NP_002831.2.

Cellular Locus

Membrane

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein amino acids 1315-1607 (cytoplasmic C-terminus) of human LAR. 97% identical in both rat and mouse. >80% identity with PTPRD and PTPRS. >50% identity with PTPRM and PTPRK.

Target

LAR

Clonality

Monoclonal

Isotype

IgG2a

Clone

N165/38 (Formerly sold as S165-38)

Conjugation

RPE

Type

Monoclonal

Applications

WB | ICC/IF

Validated Applications

WB,IHC,ICC/IF

Field of Research

Cell Signaling | Post-translational Modifications | Phosphorylation | Neuroscience | Cell Markers

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-443 was sufficient for detection of LAR/PTPRF in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.46mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Streuli M. M., et al. (1992) EMBO Journal, 11(3), 897-907. 2. Mooney R.A. and C.M LeVea. (2003) Current Topics in Medicinal Chemistry, 3(7), 809-819. 3. Chagnon M.J., et al. (2004) Biochemistry and Cell Biology, 82(6), 664-675. 4. Ahmad F.F., and B.J. Goldstein (1997) Journal of Biological Chemistry, 272(1), 448-457.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Specificity

Detects ~85kDa (full length protein is 210 kDa – smaller due to proteolysis into P-subunit containing transmembrane and intracellular domains.

Background Reference 01

1. Streuli M. M., et al. (1992) EMBO Journal, 11 (3), 897-907. 2. Mooney R.A. and C.M LeVea. (2003) Current Topics in Medicinal Chemistry, 3 (7), 809-819. 3. Chagnon M.J., et al. (2004) Biochemistry and Cell Biology, 82 (6), 664-675. 4. Ahmad F.F., and B.J. Goldstein (1997) Journal of Biological Chemistry, 272 (1), 448-457.