AMIGO-1 Antibody

Mouse Anti-Mouse AMIGO-1 Monoclonal IgG1

Product Specifications

Background

The amphoterin-induced gene and ORF (AMIGO) family of proteins consists of AMIGO1, AMIGO2 and AMIGO3. All three members are single pass type I membrane proteins that contain several leucine-rich repeats, one IgG domain and a transmembrane domain. The AMIGO proteins are specifically expressed on fiber tracts of neuronal tissues and participate in their formation. They can form complexes with each other, but can also self-bind. AMIGO1, also designated Alivin2, promotes growth and fasciculation of neurites and plays a role in myelination and fasciculation of developing neural axons. In cerebellar neurons, AMIGO2 (Alivin1) is crucial for depolarization-dependent survival. Similar to AMIGO1 and AMIGO2, AMIGO3 (Alivin3) plays a role in hemophilic and/or heterophilic cell-cell interaction and signal transduction.

CAS Number

9007-83-4

Specifications

Detects ~60-80kDa depending on maturity/glycosylation.

Product Name Alternative

AMIGO 1 Antibody, AMIGO1 Antibody, Adhesion molecule with Ig like domain 1 Antibody, Amphoterin-induced protein 1 Antibody, Alivin-2 Antibody, Alivin 2 Antibody, Ali2 Antibody, AMIGO Antibody, KIAA1163 Antibody, Amphoterin induced gene and ORF (Amigo) Antibody, Amphoterin induced protein 1 Antibody, MGC25558 Antibody, OTTHUMP00000013379 Antibody, RP23 89M15.6 Antibody

Synonyms

AMIGO1 Antibody, Clone L86/36

UNSPSC

12352203

Gene ID

229715

Swiss Prot

Q80ZD8

Accession Number

NP_001004293.1

Cellular Locus

Cell Membrane | Cell Projection | Axon

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein amino acids 394-492 (cytoplasmic C-terminus) of mouse AMIGO-1

Target

AMIGO1

Clonality

Monoclonal

Isotype

IgG1

Clone

L86/33 (Formerly sold as S86-36)

Conjugation

Unconjugated

Validated Applications

WB,IHC,ICC/IF

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-438 was sufficient for detection of AMIGO-1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Kuja-Panula J., Kiiltomäki M., Yamashiro T., Rouhiainen A. and Rauvala H. (2003) J. Cell Biol. 160: 963-973. 2. Clark H.F., et al. (2003) Genome Res. 13: 2265-2270. 3. On, T., Sekino-Suzuki N., Kikkawa Y., Yonekawa H. and Kawashima S. (2003) J. Neurosci. 23: 5887-5896. 4. Chen Y., Aulia S., Li L. and Tang B.L. (2006) Brain Res. Brain Res. Rev. 51: 265-274.