Notch1 Antibody, Clone N253/32: RPE

Mouse Anti-Mouse Notch1 Monoclonal IgG1

Product Specifications

Background

Notch1 is a transmembrane receptor involved in cell fate determination, synaptic plasticity, and neurogenesis. Following ligand binding, Notch1 undergoes sequential proteolytic cleavages, culminating in the release of the Notch intracellular domain (NICD), which translocates to the nucleus to regulate gene expression. In the nervous system, Notch1 signaling influences neural stem cell maintenance, differentiation, and response to injury. Aberrant Notch1 activation has been implicated in neurodegenerative diseases, where it may contribute to gliosis, inflammation, and impaired neurogenesis. Modulating Notch1 signaling could offer therapeutic strategies for promoting neural repair and mitigating neurodegenerative progression.

CAS Number

9007-83-4

Specifications

Detects >270kDa. Does not cross-react with Notch 2 or Notch3.

Product Name Alternative

Neurogenic locus notch homolog protein 1, hN1, Translocation associated notch protein, TAN1, TAN-1, Motch A, mT14, p300, Motch, Mis6, Neurogenic locus notch protein homolog, NICD, Notch 1 intracellular domain, Notch gene homolog 1 (Drosophila), Notch homolog 1, translocation-associated (Drosophila), NOTCH Drosophila, homolog of 1, Translocation associated notch homolog, Translocation associated notch protein TAN 1, xotch

Synonyms

Notch1 Antibody, Clone N253/32: RPE

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

18128

Swiss Prot

Q01705

Accession Number

NP_032740.3

Cellular Locus

Cell Membrane | Nucleus

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1

Target

Notch1

Clonality

Monoclonal

Isotype

IgG1

Clone

N253/32 (Formerly sold as S253-32)

Conjugation

RPE

Type

Monoclonal

Applications

WB | ICC/IF

Validated Applications

WB,IHC,ICC/IF

Field of Research

Neuroscience | Cell Signaling | Epigenetics and Nuclear Signaling | cardiovascular System | Heart

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-430 was sufficient for detection of Notch1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.46mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Swiatek P.J., et al. (1994) Genes Dev. 8: 707-719. 2. Simpson P. (1994) The Notch Receptors. Austin, TX: R.G. Landes Company. 3. Lindsell C.E., et al. (1995) Cell 80: 909-917. 4. Uyttendaele H., et al. (1996) Development 122: 2251-2259. 5. Girard L., et al. (1996) Genes Dev. 10: 1930-1944.