MGluR1/5 Antibody, Clone N75/3: HRP

Mouse Anti-Rat mGluR1/5 Glutamate Receptor Monoclonal IgG2A

Product Specifications

Background

MGluR5 (metabotropic glutamate receptor 5) is a subtype of glutamate receptor that regulates synaptic transmission and plasticity via G-protein-coupled signaling. It is predominantly expressed in the hippocampus, cortex, and striatum—regions vulnerable to neurodegeneration. Aberrant mGluR5 activity has been linked to excitotoxicity, calcium dysregulation, and neuroinflammation, contributing to the pathophysiology of Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis (ALS) . mGluR5 also interacts with amyloid precursor protein and tau, influencing their aggregation and toxicity. Pharmacological modulation of mGluR5 has shown promise in preclinical models, where antagonists reduce neuronal loss and improve cognitive outcomes. As a key regulator of glutamatergic tone, mGluR5 is a compelling target for therapeutic intervention in neurodegenerative disorders characterized by synaptic dysfunction and excitatory imbalance.

CAS Number

9007-83-4

Specifications

Detects ~130kDa (both mGluR1 and mGluR5).

Product Name Alternative

MGluR5, mGlu5, GRM5, GPRC1E, Metabotropic glutamate receptor 5, Metabotropic glutamate receptor 5 variant F, Metabotropic glutamate receptor 5 variant G, Metabotropic glutamate receptor 5 variant H, Glutamate receptor metabotropic 5, mGluR5a, mGluR5b, mGluR, Glutamate receptor metabotropic 1, mGlu1, mGluR1, GRM1, GRM 1, GRM1A, GRM1 alpha, GRM1_HUMAN, GPRC1A, Metabotropic glutamate receptor 1, mGluR1 alpha

Synonyms

mGluR1/5 Antibody, Clone N75/33: HRP

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

24418

Swiss Prot

P31424

Accession Number

NP_058708.1

Cellular Locus

Cell Membrane

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein amino acids 824-1203 (cytoplasmic C-terminus) of rat mGluR5b

Target

mGluR5

Clonality

Monoclonal

Isotype

IgG2A

Clone

N75/3 (Formerly sold as S75-3)

Conjugation

HRP

Type

Monoclonal

Applications

WB | IHC | ICC/IF | IP

Validated Applications

WB,IHC,ELISA

Field of Research

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-419 was sufficient for detection of mGluR1/5 glutamate receptor in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), IHC (1:1000), ICC/IF (1:1000) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Mayer M.L. (2005) Current Opinion in Neurobio. 15(3): 282-288. 2. Keinane K., et al. (1990) Science. 249: 556-560.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Specificity

Detects ~130kDa (both mGluR1 and mGluR5) .

Background Reference 01

1. Mayer M.L. (2005) Current Opinion in Neurobio. 15 (3) : 282-288. 2. Keinane K., et al. (1990) Science. 249: 556-560.

Species

Human | Mouse | Rat

Location

Cell Membrane

Isotype

IgG2A

Immunogen Species

Rat

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