NALCN Antibody, Clone N187/7: APC

Mouse Anti-Rat NALCN Monoclonal IgG1

Product Specifications

Background

NALCN (sodium leak channel non-selective protein), also known as CanIon or VGCNL1, is a voltage-independent, nonselective cation channel that plays a critical role in maintaining neuronal excitability. Widely expressed in the central nervous system and highly conserved across mammals, NALCN contributes to the background sodium leak conductance in neurons, regulating resting membrane potential and spontaneous firing. Activated by neuropeptides such as substance P via the NK-1 receptor, NALCN is permeable to sodium, potassium, and calcium ions. Its function is essential for respiratory rhythm generation, as evidenced by knockout mouse models that exhibit disrupted breathing and neonatal lethality. Dysregulation of NALCN has been implicated in neurodevelopmental disorders, epilepsy, and neurodegenerative conditions where altered excitability and ion homeostasis are central features. Given its role in basal neuronal activity and survival, NALCN represents a promising target for therapeutic intervention in diseases characterized by impaired excitability and synaptic dysfunction.

CAS Number

9007-83-4

Specifications

Detects ~200kDa.

Product Name Alternative

NALCN, VGCNL1, CanIon, Sodium leak channel non-selective protein, Voltage gated channel-like protein 1, A530023G15Rik, bA430M15.1, FLJ23913, FLJ44659, FLJ44764, Four repeat voltage gated ion channel, MGC74524, Putative 4 repeat voltage gated ion channel

Synonyms

NALCN Antibody, Clone N187/7: APC

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

266760

Swiss Prot

Q6Q760

Accession Number

NP_705894.1

Cellular Locus

Membrane

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein amino acids 1659-1738 (cytoplasmic C-terminus) of rat NALCN

Target

NALCN

Clonality

Monoclonal

Isotype

IgG1

Clone

N187/7 (Formerly sold as S187-7)

Conjugation

APC

Type

Monoclonal

Applications

WB | ICC/IF

Validated Applications

IHC,ICC/IF

Field of Research

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-417 was sufficient for detection of NALCN in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.46mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Lee, J.H., Cribbs, L.L. and Perez-Reyes, E. (1999)FEBS Lett. 445: 231-236. 2. Lu, B., Su, Y., Das, S., Liu, J., Xia, J. and Ren, D. (2007) Cell 129: 371-383. 3. Jospin, M., et al. (2007) Curr. Biol. 17: 1595-1600. 4. Gaultier, C. and Gallego, J. (2008) Appl. Physiol. 104: 1522-1530. 5. Yeh, E., et al. (2008) PLoS Biol. 6: e55.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Specificity

Detects ~200kDa.

Background Reference 01

1. Lee, J.H., Cribbs, L.L. and Perez-Reyes, E. (1999) FEBS Lett. 445: 231-236. 2. Lu, B., Su, Y., Das, S., Liu, J., Xia, J. and Ren, D. (2007) Cell 129: 371-383. 3. Jospin, M., et al. (2007) Curr. Biol. 17: 1595-1600. 4. Gaultier, C. and Gallego, J. (2008) Appl. Physiol. 104: 1522-1530. 5. Yeh, E., et al. (2008) PLoS Biol. 6: e55.