Dicer Antibody, Clone N167/7: Biotin

Mouse Anti-Mouse Dicer Monoclonal IgG1

Product Specifications

Background

Dicer is a ribonuclease III enzyme essential for the biogenesis of microRNAs (miRNAs), which regulate gene expression post-transcriptionally. It processes precursor miRNAs into mature ~22-nucleotide miRNAs that guide the RNA-induced silencing complex (RISC) to target mRNAs. In the nervous system, Dicer is critical for neuronal differentiation, synaptic plasticity, and stress responses. Dysregulation of Dicer or miRNA pathways has been implicated in neurodegenerative diseases, including Alzheimer’s, ALS, and Huntington’s disease. Loss of Dicer function leads to widespread transcriptomic imbalance and neuronal vulnerability, making it a key player in epigenetic regulation and a potential therapeutic target in neurodegeneration.

CAS Number

9007-83-4

Specifications

Detects ~215kDa.

Product Name Alternative

Dicer1, DCR, DCR1, DCR-1, Double-strand-specific ribonuclease, Helicase with RNase motif, HERNA, Helicase MOI, KIAA0928, Endoribonuclease Dicer

Synonyms

Dicer Antibody, Clone N167/7: Biotin

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

192119

Swiss Prot

Q8R418

Accession Number

NP_683750.2

Cellular Locus

Cytoplasm

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein amino acids 1638-1899 of mouse Endoribonuclease Dicer

Target

Dicer

Clonality

Monoclonal

Isotype

IgG1

Clone

N167/7 (Formerly sold as S167-7)

Conjugation

Biotin

Type

Monoclonal

Applications

WB | ICC/IF

Validated Applications

WB,IHC,ICC/IF |ELISA

Field of Research

Cell Signaling | Epigenetics and Nuclear Signaling

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-416 was sufficient for detection of Dicer in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Hutvágner G. and Zamore P.D. (2002) Science 297: 2056-60. 2. Lee Y., et al. (2003) Nature 425: 415-9. 3. Diederichs S. and Haber, D.A. (2007) Cell 131: 1097-108.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Specificity

Detects ~215kDa.

Background Reference 01

1. Hutvágner G. and Zamore P.D. (2002) Science 297: 2056-60. 2. Lee Y., et al. (2003) Nature 425: 415-9. 3. Diederichs S. and Haber, D.A. (2007) Cell 131: 1097-108.