Clcn3 Antibody, Clone N258/5: Biotin

Mouse Anti-Rat Clcn3 Monoclonal IgG1

Product Specifications

Background

ClC-3 (ClCN3) is a voltage-gated chloride/proton exchanger that plays a critical role in regulating endosomal and synaptic vesicle acidification. By mediating Cl⁻/H⁺ exchange, ClC-3 contributes to vesicle trafficking, neurotransmitter release, and membrane excitability—key processes in neuronal communication and plasticity. In the brain, ClC-3 is expressed in neurons and glial cells, where it influences synaptic vesicle cycling and endolysosomal function. Dysregulation of ClC-3 has been linked to impaired short-term memory, altered neuronal excitability, and neurodegenerative phenotypes in animal models. Its modulation by protein kinase C suggests a role in activity-dependent signaling pathways. Given its involvement in vesicle dynamics and ion homeostasis, ClC-3 is emerging as a potential target in neurodegenerative disease research, particularly in disorders characterized by synaptic dysfunction and lysosomal impairment.

CAS Number

9007-83-4

Specifications

Detects ~90kDa. Does not cross-react with Clcn4 or Clcn5 (based on KO validation results).

Product Name Alternative

CIC3, CLC3, CLCN 3, Chloride channel 3, Chloride channel protein 3, Chloride channel, voltage-sensitive 3, Chloride transporter ClC 3, DKFZp564I0463, H (+) /Cl (-) exchange transporter 3, Chloride transporter ClC-3, ClC-3

Synonyms

ClCN3 Antibody, Clone N258/5: Biotin

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

84360

Swiss Prot

P51792

Accession Number

NP_445815.2

Cellular Locus

Membrane | Endosome | Endosome membrane | Cytoplasmic Vesicle | Secretory Vesicle Membrane

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthetic peptide amino acids 98-115 (cytoplasmic N-terminus) of rat Clcn3

Target

ClCN3

Clonality

Monoclonal

Isotype

IgG1

Clone

N258/5 (Formerly sold as S258-5)

Conjugation

Biotin

Type

Monoclonal

Applications

WB | IHC | ICC/IF

Validated Applications

WB,IHC,ICC/IF |ELISA

Field of Research

Neuroscience | Pumps/Transporters | Ion Pumps/Transporters | Cell Signaling

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-408 was sufficient for detection of Clcn3 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), IHC (1:1000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. www.phosphosite.org/proteinAction.do?id=5088&showAllSites=true

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Specificity

Detects ~90kDa. Does not cross-react with Clcn4 or Clcn5 (based on KO validation results) .

Background Reference 01

1. www.phosphosite.org/proteinAction.do?id=5088&showAllSites=true

Species

Human | Mouse | Rat

Location

Membrane | Endosome | Endosome membrane | Cytoplasmic Vesicle | Secretory Vesicle Membrane

Isotype

IgG1

Immunogen Species

Rat

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