Clcn3 Antibody, Clone N258/5

Mouse Anti-Rat Clcn3 Monoclonal IgG1

Product Specifications

Background

ClC-3 (ClCN3) is a voltage-gated chloride/proton exchanger that plays a critical role in regulating endosomal and synaptic vesicle acidification. By mediating Cl⁻/H⁺ exchange, ClC-3 contributes to vesicle trafficking, neurotransmitter release, and membrane excitability—key processes in neuronal communication and plasticity. In the brain, ClC-3 is expressed in neurons and glial cells, where it influences synaptic vesicle cycling and endolysosomal function. Dysregulation of ClC-3 has been linked to impaired short-term memory, altered neuronal excitability, and neurodegenerative phenotypes in animal models. Its modulation by protein kinase C suggests a role in activity-dependent signaling pathways. Given its involvement in vesicle dynamics and ion homeostasis, ClC-3 is emerging as a potential target in neurodegenerative disease research, particularly in disorders characterized by synaptic dysfunction and lysosomal impairment.

CAS Number

9007-83-4

Specifications

Detects ~90kDa. Does not cross-react with Clcn4 or Clcn5 (based on KO validation results).

Product Name Alternative

CIC3, CLC3, CLCN 3, Chloride channel 3, Chloride channel protein 3, Chloride channel, voltage-sensitive 3, Chloride transporter ClC 3, DKFZp564I0463, H (+) /Cl (-) exchange transporter 3, Chloride transporter ClC-3, ClC-3

Synonyms

ClCN3 Antibody, Clone N258/5

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

84360

Swiss Prot

P51792

Accession Number

NP_445815.2

Cellular Locus

Membrane | Endosome | Endosome membrane | Cytoplasmic Vesicle | Secretory Vesicle Membrane

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthetic peptide amino acids 98-115 (cytoplasmic N-terminus) of rat Clcn3

Target

ClCN3

Clonality

Monoclonal

Isotype

IgG1

Clone

N258/5 (Formerly sold as S258-5)

Conjugation

Unconjugated

Type

Monoclonal

Applications

WB | IHC | ICC/IF

Validated Applications

WB,IHC,ICC/IF

Field of Research

Neuroscience | Pumps/Transporters | Ion Pumps/Transporters | Cell Signaling

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-408 was sufficient for detection of Clcn3 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), IHC (1:1000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. www.phosphosite.org/proteinAction.do?id=5088&showAllSites=true

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

-20ºC

Specificity

Detects ~90kDa. Does not cross-react with Clcn4 or Clcn5 (based on KO validation results) .

Background Reference 01

1. www.phosphosite.org/proteinAction.do?id=5088&showAllSites=true

Species

Human | Mouse | Rat

Location

Membrane | Endosome | Endosome membrane | Cytoplasmic Vesicle | Secretory Vesicle Membrane

Isotype

IgG1

Immunogen Species

Rat

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