Ankyrin G Antibody, Clone N106/20: RPE

Ankyrin-G (ANK3) is a critical scaffolding protein that anchors integral membrane proteins to the spectrin-actin cytoskeleton, ensuring the structural and functional integrity of excitable cells, particularly neurons. It binds to the β-spectrin subunit and a wide array of membrane proteins, including voltage-gated sodium channels, potassium channels, and cell adhesion molecules, orchestrating their precise localization at the axon initial segment (AIS) and nodes of Ranvier. In the central nervous system, Ankyrin-G plays a pivotal role in maintaining neuronal polarity, regulating action potential initiation, and preserving synaptic stability. Disruption of Ankyrin-G function leads to mislocalization of ion channels and cytoskeletal disorganization, contributing to impaired neuronal signaling. Emerging evidence links ANK3 mutations and dysregulation to a spectrum of neuropsychiatric and neurodegenerative disorders, including bipolar disorder, schizophrenia, epilepsy, and Alzheimer’s disease. In Alzheimer’s models, Ankyrin-G dysfunction has been associated with AIS disassembly and increased neuronal vulnerability, suggesting a mechanistic link between cytoskeletal breakdown and neurodegeneration. Given its central role in neuronal architecture and excitability, Ankyrin-G is increasingly recognized as a potential biomarker and therapeutic target in neurodegenerative disease research. Understanding its molecular interactions and regulatory mechanisms is essential for developing strategies to preserve neuronal function and prevent disease progression.

Ankyrin G, Ankyrin-G, Ankyrin-3, Ankyrin 3, ANK3, ANK-3, Ankyrin 3 (G), Ankyrin G119, Ankryin G119, Brain-specific ankyrin G, CHANK3, Node of Ranvier (ankyrin G), Node of Ranvier, FLJ45464, OTTHUMP00000217458, OTTHUMP00000217575, RP11-369L1.1

Fusion protein 1000 C-terminal amino acids of human Ankyrin G (also known as ANK-3 or ankyrin-3) encompassing all of Ankyrin G with the exception of Ankyrin repeats

1 µg/ml of SMC-404 was sufficient for detection of Ankyrin-G in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.