MMP9 Antibody, Clone L51/82: ATTO 488

Mouse Anti-Rat MMP9 Monoclonal IgG2a

Product Specifications

Background

Matrix metalloproteinase 9 (MMP9) is an extracellular endopeptidase involved in the degradation of the extracellular matrix (ECM) . In the central nervous system, MMP9 regulates synaptic remodeling, neuroinflammation, and blood-brain barrier permeability. Elevated MMP9 activity has been observed in neurodegenerative diseases such as Alzheimer’s disease, multiple sclerosis, and amyotrophic lateral sclerosis (ALS) . MMP9 contributes to neuronal injury by promoting ECM breakdown, facilitating leukocyte infiltration, and activating pro-inflammatory cytokines. MMP9 is synthesized as a latent proenzyme and requires proteolytic cleavage for activation. Its expression is tightly regulated at the transcriptional level and by endogenous inhibitors such as TIMPs (tissue inhibitors of metalloproteinases) . Targeting MMP9 activity offers therapeutic potential for preserving blood-brain barrier integrity, reducing neuroinflammation, and limiting tissue damage in neurodegenerative conditions.

CAS Number

9007-83-4

Specifications

Detecs ~92kDa and ~82kDa (pro and active forms).

Product Name Alternative

MMP9, MMP-9, Matrix metalloproteinase 9, CLG4B, 82kDa matrix metalloproteinase-9, Collagenease type 4 beta, GELB, Macrophage gelatinase, MANDP2, Type V collagenase, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B

Synonyms

MMP9 Antibody, Clone L51/82: ATTO 488

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

81687

Swiss Prot

P50282

Accession Number

NP_112317.1

Cellular Locus

Extracellular Matrix

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein amino acids 1-708 (full length) of rat MMP9

Target

MMP9

Clonality

Monoclonal

Isotype

IgG2a

Clone

L51/82 (Formerly sold as S51-82)

Conjugation

ATTO 488

Type

Monoclonal

Applications

WB | IHC | ICC/IF | IP | FCM

Validated Applications

IHC,ICC/IF

Field of Research

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-396 was sufficient for detection of MMP9 in 20 µg of COS-1 cells (lysate) transfected with human MMP9 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Hirose Y., et al. (2008) Am J Hum Genet. 82(5): 1122-1129. 2. Coussens L.M., et al. (2002) Science 295: 2387-2391. 3. Sternlicht M.D., et al. (1999) Cell 98: 137-146. 4. Vu T.H., et al. (1998) Cell 93: 411-422. 5. Nagase H., et al. (1990) Biochemistry 29: 5783-5789.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Specificity

Detecs ~92kDa and ~82kDa (pro and active forms) .

Background Reference 01

1. Hirose Y., et al. (2008) Am J Hum Genet. 82 (5) : 1122-1129. 2. Coussens L.M., et al. (2002) Science 295: 2387-2391. 3. Sternlicht M.D., et al. (1999) Cell 98: 137-146. 4. Vu T.H., et al. (1998) Cell 93: 411-422. 5. Nagase H., et al. (1990) Biochemistry 29: 5783-5789.