VGLUT2 Antibody, Clone N29/29: HRP

Mouse Anti-Rat VGLUT2 Monoclonal IgG1

Product Specifications

Background

Vesicular Glutamate Transporter 2 (VGLUT2), encoded by SLC17A6, is an ATP-dependent, chloride-sensitive transporter essential for loading glutamate into synaptic vesicles. Alongside VGLUT1 (BNPI) and VGLUT3, VGLUT2 plays a pivotal role in excitatory neurotransmission. Its expression is regionally distinct and complementary to VGLUT1: while VGLUT1 predominates in telencephalic regions, VGLUT2 is highly expressed in the diencephalon and lower brainstem—areas critical for autonomic control, sensory integration, and motor coordination. VGLUT2 is also expressed in rat pinealocytes and pancreatic α and β cells, where it responds to glucose levels, suggesting a broader role in neuroendocrine signaling. This transporter is particularly relevant in the context of neurodegenerative diseases, as its dysregulation has been implicated in conditions such as Parkinson’s disease, amyotrophic lateral sclerosis (ALS), and Huntington’s disease. Its involvement in glutamate homeostasis and excitotoxicity positions VGLUT2 as a key molecular target for understanding disease mechanisms and developing therapeutic interventions. Although VGLUT3 shares approximately 72% sequence homology with VGLUT2 and VGLUT1, each isoform exhibits distinct spatial and functional profiles. VGLUT2’s unique distribution and regulatory mechanisms make it a critical focus in neuroscience research, particularly in studies of synaptic plasticity, circuit dysfunction, and neurodegeneration.

CAS Number

9007-83-4

Specifications

Detects ~60kDa.

Product Name Alternative

VGLUT2, VGluT2, VGLU2, SLC17A6, Solute Carrier Family 17 Member 6, Solute Carrier Family 17 (Sodium-Dependent Inorganic Phosphate Cotransporter) Member 6, Solute Carrier Family 17 (Vesicular Glutamate Transporter) Member 6, Vesicular Glutamate Transporter 2, DNPI, Differentiation-Associated Na (+) -Dependent Inorganic Phosphate Cotransporter, Differentiation-Associated Na-Dependent Inorganic Phosphate Cotransporter, Differentiation-Associated BNPI, Sodium-Dependent Inorganic Phosphate Cotransporter

Synonyms

VGLUT2 Antibody, Clone N29/29: HRP

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

84487

Swiss Prot

Q9JI12

Accession Number

NP_445879.1

Cellular Locus

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGlut2

Target

VGLUT2

Clonality

Monoclonal

Isotype

IgG1

Clone

N29/29 (Formerly sold as S29-29)

Conjugation

HRP

Type

Monoclonal

Applications

WB | IHC | ICC/IF

Validated Applications

WB,IHC,ELISA

Field of Research

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-395 was sufficient for detection of VGLut2 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Aihara Y., et al. (2000) J Neurochem. 74: 2622-2625. 2. Kaneko T., and Fujiyama F. (2002) Neurosci Res. 42: 243-250. 3. Gras C., et al. (2002) J Neurosci. 22: 5442-5451. 4. Takamori S., Malherbe P., Broger C., and Jahn R. (2002) EMBO Rep. 3: 798-803. 5. Morimoto R., Hayashi M., Yatsushiro S., Otsuka M., Yamamoto A., and Moriyama Y. (2003) J Neurochem. 84: 382-391. 6. Bai L., Zhang X., and Ghishan F.K. (2003) Am. J. Physiol. Gastrointest. Liver Physiol. 284: G808-814.