VGLUT2 Antibody, Clone N29/29

Vesicular Glutamate Transporter 2 (VGLUT2), encoded by SLC17A6, is an ATP-dependent, chloride-sensitive transporter essential for loading glutamate into synaptic vesicles. Alongside VGLUT1 (BNPI) and VGLUT3, VGLUT2 plays a pivotal role in excitatory neurotransmission. Its expression is regionally distinct and complementary to VGLUT1: while VGLUT1 predominates in telencephalic regions, VGLUT2 is highly expressed in the diencephalon and lower brainstem—areas critical for autonomic control, sensory integration, and motor coordination. VGLUT2 is also expressed in rat pinealocytes and pancreatic α and β cells, where it responds to glucose levels, suggesting a broader role in neuroendocrine signaling. This transporter is particularly relevant in the context of neurodegenerative diseases, as its dysregulation has been implicated in conditions such as Parkinson’s disease, amyotrophic lateral sclerosis (ALS), and Huntington’s disease. Its involvement in glutamate homeostasis and excitotoxicity positions VGLUT2 as a key molecular target for understanding disease mechanisms and developing therapeutic interventions. Although VGLUT3 shares approximately 72% sequence homology with VGLUT2 and VGLUT1, each isoform exhibits distinct spatial and functional profiles. VGLUT2’s unique distribution and regulatory mechanisms make it a critical focus in neuroscience research, particularly in studies of synaptic plasticity, circuit dysfunction, and neurodegeneration.

VGLUT2, VGluT2, VGLU2, SLC17A6, Solute Carrier Family 17 Member 6, Solute Carrier Family 17 (Sodium-Dependent Inorganic Phosphate Cotransporter) Member 6, Solute Carrier Family 17 (Vesicular Glutamate Transporter) Member 6, Vesicular Glutamate Transporter 2, DNPI, Differentiation-Associated Na (+) -Dependent Inorganic Phosphate Cotransporter, Differentiation-Associated Na-Dependent Inorganic Phosphate Cotransporter, Differentiation-Associated BNPI, Sodium-Dependent Inorganic Phosphate Cotransporter

1 µg/ml of SMC-395 was sufficient for detection of VGLut2 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

1. Aihara Y., et al. (2000) J Neurochem. 74: 2622-2625. 2. Kaneko T., and Fujiyama F. (2002) Neurosci Res. 42: 243-250. 3. Gras C., et al. (2002) J Neurosci. 22: 5442-5451. 4. Takamori S., Malherbe P., Broger C., and Jahn R. (2002) EMBO Rep. 3: 798-803. 5. Morimoto R., Hayashi M., Yatsushiro S., Otsuka M., Yamamoto A., and Moriyama Y. (2003) J Neurochem. 84: 382-391. 6. Bai L., Zhang X., and Ghishan F.K. (2003) Am. J. Physiol. Gastrointest. Liver Physiol. 284: G808-814.

1. Aihara Y., et al. (2000) J Neurochem. 74: 2622-2625. 2. Kaneko T., and Fujiyama F. (2002) Neurosci Res. 42: 243-250. 3. Gras C., et al. (2002) J Neurosci. 22: 5442-5451. 4. Takamori S., Malherbe P., Broger C., and Jahn R. (2002) EMBO Rep. 3: 798-803. 5. Morimoto R., Hayashi M., Yatsushiro S., Otsuka M., Yamamoto A., and Moriyama Y. (2003) J Neurochem. 84: 382-391. 6. Bai L., Zhang X., and Ghishan F.K. (2003) Am. J. Physiol. Gastrointest. Liver Physiol. 284: G808-814.