Sodium Iodide Symporter Antibody, Clone 14F: HRP

Mouse Anti-Human Sodium-Iodide Symporter Monoclonal IgG1

Product Specifications

Background

The Sodium Iodide Symporter (NIS), encoded by the SLC5A5 gene, is a transmembrane glycoprotein responsible for actively transporting iodide ions into thyroid epithelial cells. This iodide uptake is a critical first step in the biosynthesis of thyroid hormones—triiodothyronine (T3) and thyroxine (T4) —which are essential regulators of metabolism, development, and neural function. While NIS is traditionally studied in the context of thyroid physiology and thyroid cancer imaging, emerging research highlights its broader relevance in neuroscience. Thyroid hormones play a pivotal role in brain development, synaptic plasticity, and neuroprotection. Dysregulation of NIS-mediated iodide transport can impair thyroid hormone synthesis, potentially contributing to cognitive decline, mood disorders, and neurodegenerative diseases such as Alzheimer’s and Parkinson’s. Recent studies suggest that altered NIS expression or function may influence local thyroid hormone availability in the brain, affecting neuronal energy metabolism and oxidative stress responses. These findings position NIS as a potential biomarker and therapeutic target in neurodegenerative disease research. Understanding the role of NIS in the neuroendocrine axis opens new avenues for exploring how peripheral thyroid dysfunction may drive central nervous system pathology.

CAS Number

9007-83-4

Specifications

Detects ~97kDa, non-glycosylated version at 68kDa. Other minor bands associated with hNIS at 160kDa, and degradation products at ~30 kDa, and ~15kDa.

Product Name Alternative

NIS, SLC5A5, solute carrier family 5, Na (+) I (-) cotransporter

Synonyms

Sodium Iodide Symporter Antibody, Clone 14F: HRP

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

6528

Swiss Prot

Q92911

Accession Number

NP_000444.1

Cellular Locus

Membrane

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Mannose binding protein hNIS fusion (AA468-643)

Target

Sodium Iodide Symporter

Clonality

Monoclonal

Isotype

IgG1

Clone

14F

Conjugation

HRP

Type

Monoclonal

Applications

WB | IHC | AM

Validated Applications

WB,IHC,ELISA

Field of Research

Neuroscience | Pumps/Transporters | Ion Pumps/Transporters | Cell Signaling | Cancer

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-390 was sufficient for detection of hNIS in 20 µg of transfected COS-7 cell membrane lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), IHC (1:1000) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Dai G., Levy O., Carrasco N. (1996) Nature. 379(6564): 458-460. 2. Snabik P.A., et al. (1997) Endocrin. 138(8): 3555-3558. 3. Dohan O., et al. (2007) Proc Natl Acad Sci USA. 104(51): 20250-20255.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Specificity

Detects ~97kDa, non-glycosylated version at 68kDa. Other minor bands associated with hNIS at 160kDa, and degradation products at ~30 kDa, and ~15kDa.

Background Reference 01

1. Dai G., Levy O., Carrasco N. (1996) Nature. 379 (6564) : 458-460. 2. Snabik P.A., et al. (1997) Endocrin. 138 (8) : 3555-3558. 3. Dohan O., et al. (2007) Proc Natl Acad Sci USA. 104 (51) : 20250-20255.