SHANK3 Antibody, Clone S69

Mouse Anti-Rat SHANK3 Monoclonal IgG2b

Product Specifications

Background

SHANK3 is a multidomain scaffolding protein located at the postsynaptic density of excitatory synapses. It anchors glutamate receptors, ion channels, and signaling proteins to the actin cytoskeleton, playing a vital role in synaptic structure and function. SHANK3 is involved in receptor tyrosine kinase signaling, including the ERK-MAPK and PI3K pathways, which are essential for synaptic plasticity and neuronal survival. Mutations or deletions in SHANK3 are strongly associated with autism spectrum disorders and Phelan-McDermid syndrome, often resulting in impaired language and social communication. In neurodegenerative disease, SHANK3 dysfunction contributes to synaptic disassembly and cognitive decline. Its role in maintaining synaptic integrity and regulating intracellular signaling makes SHANK3 a critical target for understanding and treating synaptic pathologies in both developmental and degenerative brain disorders.

CAS Number

9007-83-4

Specifications

Detects ~190kDa. No cross-reactivity against Shank1 or Shank2.

Product Name Alternative

AI841104, DEL22q13.3, KIAA1650, Proline rich synapse associated protein 2, Proline-rich synapse-associated protein 2, ProSAP2, PSAP2, SH3 and multiple ankyrin repeat domains 3, SH3 and multiple ankyrin repeat domains protein 3, SH3/ankyrin domain gene 3, SHAN3_HUMAN, Shank postsynaptic density protein, Shank3, Shank3b, SPANK 2, SPANK2

Synonyms

SHANK3 Antibody, Clone S69

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

59312

Swiss Prot

Q9JLU4

Accession Number

NP_067708.1

Cellular Locus

Cytoplasm | Cell Junction | Synapse | Postsynaptic Cell Membrane | Postsynaptic Density

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthetic peptide amino acids 840-857 of rat Shank3

Target

SHANK3

Clonality

Monoclonal

Isotype

IgG2b

Clone

S69

Conjugation

Unconjugated

Type

Recombinant Monoclonal

Applications

WB | IHC | ICC/IF | IP | AM

Validated Applications

WB,IHC,ICC/IF,IP,AM

Field of Research

Neuroscience | Cell Markers | Neuron Markers | Postsynaptic Markers

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-336 was sufficient for detection of Shank3 in 10 µg COS cell lysate transiently transfected with Shank3 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), IHC (1:100), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Sheng M., and Kim E. (2000) Journal of Cell Science. 113: 1851-1856. 2. Schuetz G., et al. (2004) JCB. 167(5): 645-952. 3. Durand C.M., et al. (2007) Nature Genetics. 39: 25-27.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

-20ºC

Specificity

Detects ~190kDa. No cross-reactivity against Shank1 or Shank2.

Background Reference 01

1. Sheng M., and Kim E. (2000) Journal of Cell Science. 113: 1851-1856. 2. Schuetz G., et al. (2004) JCB. 167 (5) : 645-952. 3. Durand C.M., et al. (2007) Nature Genetics. 39: 25-27.