CavBeta2 Antibody, Clone N8b/1: RPE

Mouse Anti-Rat CavBeta2 Monoclonal IgG1

Product Specifications

Background

The Cav Beta 2 subunit is a critical auxiliary component of voltage-gated calcium channels (VGCCs), playing a central role in neuronal excitability and synaptic signaling. It facilitates the trafficking of the pore-forming alpha1 subunit to the plasma membrane by masking an endoplasmic reticulum (ER) retention signal, ensuring proper channel localization and surface expression. This process is essential for the functional assembly of VGCCs at the neuronal membrane. Beyond trafficking, Cav Beta 2 directly influences the biophysical properties of calcium channels, including voltage sensitivity, activation kinetics, and inactivation dynamics. These properties are crucial for regulating calcium influx, which in turn governs neurotransmitter release, gene expression, and neuronal survival. Dysregulation of Cav Beta 2 function has been implicated in several neurodegenerative diseases, where altered calcium homeostasis contributes to synaptic dysfunction, excitotoxicity, and progressive neuronal loss. Understanding the molecular mechanisms by which Cav Beta 2 modulates VGCCs offers valuable insights into the pathophysiology of disorders such as Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis (ALS) . As a modulator of both channel trafficking and function, Cav Beta 2 represents a promising target for therapeutic intervention aimed at restoring calcium signaling balance in neurodegenerative conditions.

CAS Number

9007-83-4

Specifications

Detects ~78 kDa. No cross reactivity against Cavβ1, Cavβ3, Cavβ4.

Product Name Alternative

Voltage-dependent L-type calcium channel subunit beta-2, Calcium channel voltage-dependent subunit beta 2, CACNB2, CAB2, Cacnlb2, cacnb2

Synonyms

CavBeta2 Antibody, Clone N8b/1: RPE

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

116600

Swiss Prot

Q8VGC3

Accession Number

NP_446303

Cellular Locus

Cell Membrane | Sarcolemma

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthetic peptide amino acids 189-205 of rat CavBeta2

Target

CavBeta2

Clonality

Monoclonal

Isotype

IgG1

Clone

N8b/1 (Formerly sold as S8B-1)

Conjugation

RPE

Type

Monoclonal

Applications

WB | IHC | ICC/IF | IP | AM

Validated Applications

WB,IHC,ICC/IF

Field of Research

Neuroscience | Ion Channels | Calcium Channels | Voltage-Gated Calcium Channels

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-332 was sufficient for detection of Cavβ2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody

Concentration

1 mg/ml

Dilution

WB (1:1000), IHC (1:1000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.46mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Dolphin A.C. (2003) J Bioenerg. Biomembr. 35: 599-620. 2. Bichet D., et al. (2000) Neuron. 25: 177-190. 3. Xie M., et al. (2007) J Cell Biol. 178(3): 489-502.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Specificity

Detects ~78 kDa. No cross reactivity against Cavβ1, Cavβ3, Cavβ4.

Background Reference 01

1. Dolphin A.C. (2003) J Bioenerg. Biomembr. 35: 599-620. 2. Bichet D., et al. (2000) Neuron. 25: 177-190. 3. Xie M., et al. (2007) J Cell Biol. 178 (3) : 489-502.