SHANK1 Antibody, Clone N22/21: RPE

Mouse Anti-Rat SHANK1 Monoclonal IgG1

Product Specifications

Background

SHANK1 is a postsynaptic density (PSD) scaffolding protein that integrates neurotransmitter receptors and signaling molecules into the cortical cytoskeleton. It plays a key role in synaptic organization, plasticity, and signal transduction at excitatory synapses. SHANK1 recruits proteins such as betaPIX and PAK to dendritic spines, regulating spine morphology and synaptic strength. It also interacts with ionotropic and metabotropic glutamate receptor complexes, contributing to synaptic signaling fidelity. Alternative splicing of SHANK1 influences its interaction network, affecting synaptic composition during development and in the adult brain. Altered SHANK1 expression or function has been linked to cognitive deficits and neuropsychiatric conditions, highlighting its relevance in neurodegenerative disease mechanisms.

CAS Number

9007-83-4

Specifications

Detects ~190-220 kDa (alternative splice variants). No cross-reactivity against Shank2 or Shank3.

Product Name Alternative

GKAP/SAPAP interacting protein, OTTHUMP00000174437, SH3 and multiple ankyrin repeat domains 1, SH3 and multiple ankyrin repeat domains protein 1, SH3/ankyrin domain gene 1, SHAN1_HUMAN, SHANK 1, Shank1, Shank1a, Somatostatin receptor interacting protein, Somatostatin receptor-interacting protein, SPANK 1, SPANK1, SSTR interacting protein, SSTR-interacting protein, SSTRIP, Synamon

Synonyms

SHANK1 Antibody, Clone N22/21: RPE

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

78957

Swiss Prot

Q9WV48

Accession Number

NP_113939.2

Cellular Locus

Cytoplasm | Cell Junction | Synapse | Postsynaptic Cell Membrane | Postsynaptic Density

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein amino acids 469-691 (SH3/PDZ domains) of rat Shank1

Target

SHANK1

Clonality

Monoclonal

Isotype

IgG1

Clone

N22/21 (Formerly sold as S22-21)

Conjugation

RPE

Type

Monoclonal

Applications

WB | IHC | AM

Validated Applications

WB,IHC,ICC/IF

Field of Research

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-329 was sufficient for detection of Shank1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), IHC (1:1000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.46mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Sheng M., and Kim E. (2000) Journal of Cell Science. 113: 1851-1856. 2. Kim J.H., et al. (2009) Exp Mol Med. 41(4): 236-242. 3. Gong Y., et al. (2009) Brain Res. 1292: 191-198.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Specificity

Detects ~190-220 kDa (alternative splice variants) . No cross-reactivity against Shank2 or Shank3.

Background Reference 01

1. Sheng M., and Kim E. (2000) Journal of Cell Science. 113: 1851-1856. 2. Kim J.H., et al. (2009) Exp Mol Med. 41 (4) : 236-242. 3. Gong Y., et al. (2009) Brain Res. 1292: 191-198.