SHANK Antibody, Clone N23b/49

Mouse Anti-Rat SHANK (pan) Monoclonal IgG1

Product Specifications

Background

SHANK proteins are a family of postsynaptic scaffolding proteins that organize receptor and signaling complexes at excitatory synapses. They contain multiple protein interaction domains, including ankyrin repeats, SH3, PDZ, and SAM domains, enabling them to link neurotransmitter receptors to the actin cytoskeleton. SHANK proteins regulate synaptic strength, dendritic spine morphology, and receptor trafficking. Disruption of SHANK-mediated scaffolding impairs glutamate receptor localization and contributes to synaptic loss—a key feature of Alzheimer’s disease. SHANK family members also influence receptor tyrosine kinase signaling and are involved in neurodevelopmental processes. Their dysregulation has been associated with autism spectrum disorders, schizophrenia, and intellectual disability, making them central to both neurodevelopmental and neurodegenerative research.

CAS Number

9007-83-4

Specifications

Detects ~160kDa. Recognizes Shank1, 2 and 3.

Product Name Alternative

Cortactin binding protein 1, Cortactin SH3 domain-binding protein, Cortactin-binding protein 1, CortBP1, CTTNBP1, GKAP/SAPAP interacting protein, GKAP/SAPAP-interacting protein, KIAA1022, Proline rich synapse associated protein 1, Proline-rich synapse-associated protein 1, ProSAP1, SH3 and multiple ankyrin repeat domains protein 2, SHAN2_RAT, Shank2, SPANK-3

Synonyms

SHANK Antibody, Clone N23b/49

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

171093

Swiss Prot

Q9QX74

Cellular Locus

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein amino acids 84-309 of rat Shank2

Target

SHANK

Clonality

Monoclonal

Isotype

IgG1

Clone

N23b/49 (Formerly sold as S23b-49)

Conjugation

Unconjugated

Type

Monoclonal

Applications

WB | IHC | IP | ICC/IF | AM

Validated Applications

WB,IHC,IP,ICC/IF,AM

Field of Research

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-327 was sufficient for detection of Shank1-4 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), IHC (1:1000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.012

Buffer

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Sheng M., and Kim E. (2000) Journal of Cell Science. 113: 1851-1856. 2. Kim J.H., et al. (2009) Exp Mol Med. 41(4): 236-242. 3. Gong Y., et al. (2009) Brain Res. 1292: 191-198.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

-20ºC

Specificity

Detects ~160kDa. Recognizes Shank1, 2 and 3.

Background Reference 01

1. Sheng M., and Kim E. (2000) Journal of Cell Science. 113: 1851-1856. 2. Kim J.H., et al. (2009) Exp Mol Med. 41 (4) : 236-242. 3. Gong Y., et al. (2009) Brain Res. 1292: 191-198.