ERp57 (GRP58) Antibody, Clone 4F9

Mouse Anti-Human ERp57 (GRP58) Monoclonal IgG1

Product Specifications

Background

ERp57, also known as PDIA3, is a thiol-disulfide isomerase involved in protein folding and quality control within the endoplasmic reticulum (ER) . In the brain, ERp57 plays a critical role in maintaining proteostasis, particularly under conditions of ER stress—a key feature of neurodegenerative diseases. ERp57 is upregulated in response to misfolded protein accumulation, as seen in Alzheimer’s, ALS, and prion diseases. It interacts with calnexin and calreticulin to assist in the proper folding of glycoproteins, including those involved in synaptic function and neuronal survival. Dysregulation of ERp57 impairs the unfolded protein response (UPR), contributing to neuronal dysfunction and cell death. Given its role in mitigating proteotoxic stress, ERp57 is emerging as a potential therapeutic target for enhancing cellular resilience in neurodegeneration.

CAS Number

9007-83-4

Specifications

Detects ~57 kDa protein.

Product Name Alternative

ERp60, ERp61, Grp57, Grp58, P58, PDIA3, PI PLC, 58 kDa glucose regulated protein, 58 kDa glucose-regulated protein, 58 kDa microsomal protein, Disulfide isomerase ER 60, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, Endoplasmic reticulum resident protein 60, ER p57, ER protein 57, ER protein 60, ERp 57, ERp57, Glucose Regulated Protein 58 Kd, GRP 57, GRP 58, GRP57, HsT17083, p58, PDIA 3, PDIA3_HUMAN, Phospholipase C alpha, Protein disulfide isomerase A3, Protein disulfide isomerase family A member 3, Protein disulfide-isomerase A3

Synonyms

ERp57 Antibody, Clone 4F9

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

2923

Swiss Prot

P30101

Accession Number

NP_005304.3

Cellular Locus

Endoplasmic Reticulum | Endoplasmic Reticulum Lumen | Melanosome

Host

Mouse

Species Reactivity

Human

Immunogen

Human recombinant ERp57 (Grp58) protein corresponding to amino acids 25 - 505 (his-tagged)

Target

ERp57

Clonality

Monoclonal

Isotype

IgG1

Clone

4F9

Conjugation

Unconjugated

Type

Monoclonal

Applications

WB | ICC/IF

Validated Applications

WB,ICC/IF

Field of Research

Cell Signaling | Protein Trafficking | ER Proteins

Purification

Protein G Purified

Limit Of Detection

A 1:1000 dilution of SMC-266 was sufficient for detection of ERp57 in 15 µg of HeLa by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000) ; ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.012

Buffer

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Herbert D.N. and Molinari M. (2007) Physiol Rev. 87: 1377-1408. 2. Williams D.B. (2005) J Cell Sci. 119: 615-623 3. Maattanen P., et al. (2006) Biochem Cell Biol. 84: 881-889. 4. Oliver J.D., et al. (1999) Mol Bio Cell. 10: 2573-2582. 5. Oliver J.D., et al. (1997) Science 275: 86-88. 6. Solda T., et al. (2006) J Biol Chem 281: 6219-6226. 7. Kimura T., et al. (2005) Biochem Biophys Research Communications. 331 (1): 224-230. 8. Chen, G., et al. (2002) Clin Cancer Res 8(7): 2298-2305. 9. Tan, P., et al. F. (2002) J Immunol 168(4): 1950-1960.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

-20ºC

Specificity

Detects ~57 kDa protein.

Background Reference 01

1. Herbert D.N. and Molinari M. (2007) Physiol Rev. 87: 1377-1408. 2. Williams D.B. (2005) J Cell Sci. 119: 615-623 3. Maattanen P., et al. (2006) Biochem Cell Biol. 84: 881-889. 4. Oliver J.D., et al. (1999) Mol Bio Cell. 10: 2573-2582. 5. Oliver J.D., et al. (1997) Science 275: 86-88. 6. Solda T., et al. (2006) J Biol Chem 281: 6219-6226. 7. Kimura T., et al. (2005) Biochem Biophys Research Communications. 331 (1) : 224-230. 8. Chen, G., et al. (2002) Clin Cancer Res 8 (7) : 2298-2305. 9. Tan, P., et al. F. (2002) J Immunol 168 (4) : 1950-1960.