N-terminal Arginylation Antibody, Clone 4D12

Mouse Anti-N-terminal Arginylation Monoclonal IgG1

Product Specifications

Background

Protein arginylation, specifically at the N-terminus, is a critical post-translational modification mediated by the enzyme ATE1. This process regulates protein turnover, stress response, and cytoskeletal organization. In neurons, N-terminal arginylation facilitates the clearance of misfolded proteins and modulates actin dynamics, both essential for synaptic integrity and plasticity. Emerging evidence suggests that dysregulation of arginylation contributes to neurodegenerative pathologies by impairing proteostasis and enhancing susceptibility to cellular stress. Arginylation also influences the unfolded protein response and autophagy, linking it to mechanisms underlying Alzheimer’s and Huntington’s disease. As a regulator of neuronal resilience and intracellular signaling, N-terminal arginylation is gaining attention as a potential therapeutic target in neurodegeneration.

CAS Number

9007-83-4

Specifications

Specific for N-terminal arginine, next to both glutamic acid and aspartic acid. Does not detect internal arginine.

Product Name Alternative

N-terminal Arginine, N-terminal Arginylation, N-terminal Arginylated, N terminal Arginine, N terminal Arginylation, N terminal Arginylated, Amino-terminal Arginine, Amino-terminal Arginylation, Amino-terminal Arginylated, Amino terminal Arginine, Amino terminal Arginylation, Amino terminal Arginylated

Synonyms

N-terminal Arginylation Antibody, Clone 4D12

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Cellular Locus

Endoplasmic Reticulum

Host

Mouse

Species Reactivity

Species Independent

Immunogen

Synthetic N-terminal arginylated peptide conjugated to KLH

Target

N-terminal Arginylation

Clonality

Monoclonal

Isotype

IgG1

Clone

4D12

Conjugation

Unconjugated

Type

Monoclonal

Applications

Validated Applications

Field of Research

Cell Signaling | Post-translational Modifications | Arginylation

Purification

Protein G Purified

Limit Of Detection

A 1:1000 dilution of SMC-264 was sufficient for detection of N-terminal Arginylation in 0.5 ug of N-terminal Arginine peptide conjugated to BSA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.