N-terminal Arginylation Antibody, Clone 4A9: HRP

Mouse Anti-N-terminal Arginylation Monoclonal IgG1

Product Specifications

Background

N-terminal arginylation is a post-translational modification catalyzed by arginyltransferase ATE1, in which an arginine residue is added to the N-terminus of proteins. This process plays a critical role in protein quality control, cellular stress responses, and neurodevelopment. In the nervous system, N-terminal arginylation regulates the degradation of misfolded or damaged proteins via the N-degron pathway, thereby maintaining proteostasis—a function essential for neuronal survival. Recent studies have linked impaired arginylation to neurodegenerative diseases such as Alzheimer’s and Parkinson’s, where accumulation of misfolded proteins and disrupted stress responses are key pathological features. Arginylation also modulates cytoskeletal dynamics and synaptic plasticity, suggesting broader roles in neuronal connectivity and cognitive function. Dysregulation of ATE1 activity has been associated with increased vulnerability to oxidative stress and endoplasmic reticulum stress, both of which are implicated in neurodegeneration. Given its multifaceted role in neuronal health, N-terminal arginylation represents a promising target for therapeutic intervention and biomarker development in neurodegenerative disease research.

CAS Number

9007-83-4

Specifications

Specific for N-terminal arginine next to glutamic acid. Does not detect N-terminal arginine next to aspartic acid or internal arginine residues.

Product Name Alternative

N-terminal Arginine, N-terminal Arginylation, N-terminal Arginylated, N terminal Arginine, N terminal Arginylation, N terminal Arginylated, Amino-terminal Arginine, Amino-terminal Arginylation, Amino-terminal Arginylated, Amino terminal Arginine, Amino terminal Arginylation, Amino terminal Arginylated

Synonyms

N-terminal Arginylation Antibody, Clone 4A9: HRP

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Cellular Locus

Endoplasmic Reticulum

Host

Mouse

Species Reactivity

Species Independent

Immunogen

Synthetic N-terminal arginylated peptide conjugated to KLH

Target

N-terminal Arginylation

Clonality

Monoclonal

Isotype

IgG1

Clone

4A9

Conjugation

HRP

Type

Recombinant Monoclonal

Applications

WB | FCM | FACS

Validated Applications

WB,IHC,ELISA

Field of Research

Cell Signaling | Post-translational Modifications | Arginylation

Purification

Protein G Purified

Limit Of Detection

A 1:1000 dilution of SMC-263 was sufficient for detection of N-terminal Arginylation in 0.5 ug of N-terminal Arginine peptide conjugated to BSA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000) ; FCM (1:50) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Saha S. and Kashina A. (2011) Dev Biol. 385(1): 1-8. 2. Deka K., et al. (2016) Cell Death Discov. 2: 16074.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Specificity

Specific for N-terminal arginine next to glutamic acid. Does not detect N-terminal arginine next to aspartic acid or internal arginine residues.

Background Reference 01

1. Saha S. and Kashina A. (2011) Dev Biol. 385 (1) : 1-8. 2. Deka K., et al. (2016) Cell Death Discov. 2: 16074.

Species

Species Independent

Location

Endoplasmic Reticulum

Isotype

IgG1

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