EndoPDI Antibody, Clone 2E7/7

Mouse Anti-Human EndoPDI Monoclonal IgG2a

Product Specifications

Background

Protein disulfide isomerase (PDI) is a thiol-disulfide oxidoreductase that facilitates protein folding and quality control in the endoplasmic reticulum (ER) . In neurons, PDI plays a protective role by preventing the accumulation of misfolded proteins and maintaining redox homeostasis. Under conditions of ER stress—common in neurodegenerative diseases—PDI expression is upregulated as part of the unfolded protein response (UPR) . However, excessive or dysfunctional PDI activity has been linked to protein aggregation and oxidative damage in Alzheimer’s, ALS, and Parkinson’s disease. PDI’s dual role in neuroprotection and pathology makes it a compelling target for therapeutic strategies aimed at modulating ER stress and proteostasis in neurodegeneration.

CAS Number

9007-83-4

Specifications

Detects ~48kDa.

Product Name Alternative

Thioredoxin domain containing 5, ERP46, UNQ364, MGC3178, FLJ21353, FLJ90810, thioredoxin related protein, endothelial protein disulphide isomeras

Synonyms

EndoPDI Antibody, Clone 2E7/7

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

81567

Swiss Prot

Q8NBS9

Accession Number

NP_001139021

Cellular Locus

Endoplasmic Reticulum | Endoplasmic Reticulum Lumen

Host

Mouse

Species Reactivity

Human

Immunogen

Synthesized peptide - 12 amino acids long (ADGEDGQDPHSK) corresponding to residues 52 - 63 of the Human EndoPDI protein

Target

PDI

Clonality

Monoclonal

Isotype

IgG2a

Clone

2E7/7

Conjugation

Unconjugated

Type

Monoclonal

Applications

WB | IHC | ICC/IF | IP | ELISA

Validated Applications

WB,IHC,ICC/IF,IP,ELISA

Field of Research

Cell Signaling | Protein Trafficking | Chaperone Proteins | Organelle Markers

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-204 was sufficient for detection of EndoPDI in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti -mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:200) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Sullivan D.C., et al. (2003) J Biol Chem. 278 (47) 47079-47088.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

-20ºC

Specificity

Detects ~48kDa.

Background Reference 01

1. Sullivan D.C., et al. (2003) J Biol Chem. 278 (47) 47079-47088.

Species

Human

Location

Endoplasmic Reticulum | Endoplasmic Reticulum Lumen

Isotype

IgG2a

Immunogen Species

Human

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