CENP-A Antibody, Clone 5A7-2E11: ATTO 488

Mouse Anti-Human CENP-A Monoclonal IgG1

Product Specifications

Background

CENPA is a histone H3 variant that defines centromeric chromatin and is essential for kinetochore assembly and chromosome segregation. It replaces canonical H3 in centromeric nucleosomes and recruits key centromere proteins such as CENP-C. While primarily studied in mitotic cells, emerging research suggests that CENPA may also play roles in post-mitotic neurons by maintaining chromatin architecture and genome stability. Disruption of centromeric integrity and epigenetic regulation has been linked to neuronal aging and neurodegenerative diseases. CENPA’s role in chromatin dynamics and nuclear organization positions it as a potential biomarker for genomic instability in neurodegeneration.

CAS Number

9007-83-4

Specifications

Detects ~18kDa. Recognizes Human CENP-A.

Product Name Alternative

CENPA, CENP-A, Centromere autoantigen A, Histone H3-like centromeric protein A

Synonyms

CENP-A Antibody, Clone 5A7-2E11: ATTO 488

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

1058

Swiss Prot

P49450

Accession Number

NP_001035891.1

Cellular Locus

Nucleus | Chromosome | Centromere | Kinetochore

Host

Mouse

Species Reactivity

Human

Immunogen

Synthetic peptide corresponding to a portion of human CENP-A

Target

CENPA

Clonality

Monoclonal

Isotype

IgG1

Clone

5A7-2E11

Conjugation

ATTO 488

Type

Monoclonal

Applications

WB | ICC/IF

Validated Applications

IHC,ICC/IF

Field of Research

Cell Signaling | Epigenetics and Nuclear Signaling

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-202 was sufficient for detection of CENPA in 20 µg of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Rieder C.L., et al. (1998) Trends Cell Biol. 8: 310-318. 2. Choo K.H. (2000) Trends Cell Biol. 10: 182-188. 3. Muro Y., et al. (2000) Clin. Exp. Immunol. 120: 218-223. 4. Howman E.V., et al. (2000) Proc. Natl. Acad. Sci. USA 97: 1148-1153.