DNMT1 Antibody, Clone 60B1220.1

Mouse Anti-Human DNMT1 Monoclonal IgG1 Kappa

Product Specifications

Background

DNMT1 (DNA methyltransferase 1) is the primary enzyme responsible for maintaining DNA methylation patterns during cell division. In the nervous system, DNMT1 regulates gene expression, synaptic plasticity, and neuronal differentiation through epigenetic mechanisms. Aberrant DNMT1 activity has been linked to neurodegenerative diseases, where altered methylation contributes to transcriptional silencing of neuroprotective genes and activation of pathogenic pathways. DNMT1 interacts with key regulatory proteins such as retinoblastoma (Rb), E2F1, and HDAC1, forming complexes that influence chromatin structure and gene repression. Loss of DNMT1 function leads to global hypomethylation, genomic instability, and impaired neuronal survival. Its role in epigenetic remodeling makes DNMT1 a promising target for therapeutic intervention and biomarker discovery in neurodegeneration.

CAS Number

9007-83-4

Specifications

Detects ~180kDa. It will cross-react with mouse DNMT1.

Product Name Alternative

DNA Mtase, DNMT, MCMT, DNA methyltransferase 1, AIM, CXXC9, DNA (cytosine-5) -methyltransferase 1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI

Synonyms

DNMT1 Antibody, Clone 60B1220.1

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

1786

Swiss Prot

P26358

Accession Number

NP_001370

Cellular Locus

Nucleus

Host

Mouse

Species Reactivity

Human,Mouse,Fish,Zebrafish

Immunogen

Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1

Target

DNMT1

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

60B1220.1

Conjugation

Unconjugated

Type

Monoclonal

Applications

WB | IHC | IP | ChIP

Validated Applications

WB,IHC,IP,ChIP

Field of Research

Cell Signaling | Post-translational Modifications | Organelle Markers

Purification

Protein G Purified

Limit Of Detection

2 µg/ml of SMC-200 was sufficient for detection of Dnmt1 in 10 µg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), IHC (1:1000) ; optimal dilutions for assays should be determined by the user.

Weight

0.012

Buffer

PBS, 50% glycerol, 0.09% sodium azide

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Bestor T., et al.(1988) J.Mol. Biol. 203: 971-983. 2. Yen R.W., Vertino P.M., Nelkin B.D., et al. (1992) Nucl. Acids Res. 20: 2287-2291. 3. Xie S., et al. (1999) Gene 236: 87-95. 4. Okano M., Bell D.W., Haber D.A. and Li E. (1999) Cell 99: 247-257. 5. Reik W. et al. (1999) J. Nat. Genet 23: 380-382. 6. Robertson K.D., et al. (2000) Nat Genet 25(3): 338-342.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

-20ºC

Specificity

Detects ~180kDa. It will cross-react with mouse DNMT1.

Background Reference 01

1. Bestor T., et al. (1988) J.Mol. Biol. 203: 971-983. 2. Yen R.W., Vertino P.M., Nelkin B.D., et al. (1992) Nucl. Acids Res. 20: 2287-2291. 3. Xie S., et al. (1999) Gene 236: 87-95. 4. Okano M., Bell D.W., Haber D.A. and Li E. (1999) Cell 99: 247-257. 5. Reik W. et al. (1999) J. Nat. Genet 23: 380-382. 6. Robertson K.D., et al. (2000) Nat Genet 25 (3) : 338-342.