MDC1 Antibody, Clone P2B11

Mouse Anti-Mouse MDC1 Monoclonal IgG1

Product Specifications

Background

Mediator of DNA Damage Checkpoint 1 (MDC1) is a key regulator of the DNA damage response (DDR), orchestrating the recruitment of repair proteins to sites of double-strand breaks. In post-mitotic neurons, where genomic integrity is critical for long-term function, MDC1 plays a vital role in maintaining neuronal viability. Dysregulation of MDC1 impairs DNA repair efficiency, contributing to the accumulation of DNA lesions observed in neurodegenerative diseases such as Alzheimer’s and Huntington’s disease. MDC1 also interacts with chromatin remodeling complexes and checkpoint kinases, linking it to broader epigenetic and cell cycle control mechanisms. As DNA damage and genomic instability are emerging hallmarks of neurodegeneration, MDC1 represents a promising target for therapeutic strategies aimed at enhancing neuronal resilience and genomic maintenance.

CAS Number

9007-83-4

Specifications

Detects ~184kDa. This antibody recognizes MDC1 at and around the N-terminus.

Product Name Alternative

MDC1, Nuclear factor with BRCT domains1, Mediator of DNA damage checkpoint 1

Synonyms

MDC1 Antibody, Clone P2B11

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

240087

Swiss Prot

Q5PSV9

Accession Number

NP_001010833.2

Cellular Locus

Nucleus

Host

Mouse

Species Reactivity

Human,Mouse,Bovine,Chimpanzee

Immunogen

GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus

Target

MDC1

Clonality

Monoclonal

Isotype

IgG1

Clone

P2B11

Conjugation

Unconjugated

Type

Monoclonal

Applications

WB | ICC/IF

Validated Applications

WB,ICC/IF

Field of Research

Cancer | Oxidative Stress | Cell Signaling | Epigenetics and Nuclear Signaling

Purification

Protein G Purified

Limit Of Detection

0.5 µg/ml of SMC-197 was sufficient for detection of MDC1 in 10 µg of HeLa cell lysate by ECL immunoblot analysis.

Concentration

1 mg/ml

Dilution

WB (1:2000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Lou Z., et al. (2004) J Biol Chem. 279(45): 46359-46362. 2. Luo K., Yuan J, Lou Z. (2011) J Biol Chem. 286(32): 28192-28199. 3. Strauss C., Halevy T., Macarov M., Argaman L., and Goldbery M. (2011) DNA Repair (Amst.). 10(8): 806-814. 4. Wilson K.A., et al. (2011) Mol Cancer Res. 9(6): 766-781.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

-20ºC

Specificity

Detects ~184kDa. This antibody recognizes MDC1 at and around the N-terminus.

Background Reference 01

1. Lou Z., et al. (2004) J Biol Chem. 279 (45) : 46359-46362. 2. Luo K., Yuan J, Lou Z. (2011) J Biol Chem. 286 (32) : 28192-28199. 3. Strauss C., Halevy T., Macarov M., Argaman L., and Goldbery M. (2011) DNA Repair (Amst.) . 10 (8) : 806-814. 4. Wilson K.A., et al. (2011) Mol Cancer Res. 9 (6) : 766-781.