GRP78 Antibody, Clone 6H4-2G7: APC

Mouse Anti-Human GRP78 Monoclonal IgG1

Product Specifications

Background

GRP78 (also known as BiP) is a 78-kDa glucose-regulated protein and a master regulator of the unfolded protein response (UPR) . As a key ER chaperone, it ensures proper protein folding, assembly, and the clearance of misfolded proteins, especially under stress conditions. Its expression is controlled by an internal ribosome entry site (IRES), which becomes highly active during cellular stress, such as heat shock or oxidative damage. GRP78 is essential for maintaining neuronal homeostasis and preventing apoptosis, making it a critical player in neurodegenerative disease contexts. In neuroscience, GRP78 has demonstrated neuroprotective effects against glutamate toxicity and oxidative stress. Its levels are notably reduced in the brains of Alzheimer’s disease patients, highlighting its potential as a biomarker and therapeutic target. Additionally, GRP78 supports embryonic development and pluripotent cell survival, further underscoring its importance in neural cell viability. However, its overexpression under extreme stress may contribute to drug resistance in neuro-oncology, adding complexity to its therapeutic modulation.

CAS Number

9007-83-4

Specifications

Detects ~78kDa.

Product Name Alternative

GRP78, GRP-78, Grp78, GRP 78, HSPA5, HSPA 5, Heat shock 70 kDa protein 5, Heat Shock 70kDa Protein 5, BiP, BIP, Binding immunoglobulin protein, Immunoglobulin heavy chain-binding protein, Immunoglobulin Heavy Chain Binding Protein, Endoplasmic reticulum lumenal Ca (2+) -binding protein grp78, Endoplasmic reticulum lumenal, Ca2+ binding protein grp78, Glucose Regulated Protein 78kDa, 78 kDa glucose-regulated protein, 78 kDa glucose regulated protein, HSCe70, MIF2, mBiP, Sez7, AL022860, AU019543, D2Wsu141e, D2Wsu17e, FLJ26106, GRP78_HUMAN

Synonyms

GRP78 Antibody, Clone 6H4-2G7: APC

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

14828

Swiss Prot

P20029

Accession Number

NP_001156906.1

Cellular Locus

Endoplasmic Reticulum | Endoplasmic Reticulum Lumen | Melanosome

Host

Mouse

Species Reactivity

Human,Mouse,Rat,Bovine,Fungi,Hamster,Monkey,Rabbit,Xenopus

Immunogen

His-tagged human GRP78

Target

GRP78

Clonality

Monoclonal

Isotype

IgG1

Clone

6H4-2G7

Conjugation

APC

Type

Monoclonal

Applications

WB | ICC/IF | ELISA

Validated Applications

IHC,ICC/IF

Field of Research

Purification

Protein G Purified

Limit Of Detection

0.5 µg/ml of SMC-196 was sufficient for detection of Grp78 in 10 µg of HeLa cell lysate by ECL immunoblot analysis.

Concentration

1 mg/ml

Dilution

WB (1:2000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.46mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Cho S., et al. (2007) Mol Cell Biol. 27(1): 368-83. 2. Yang Y., et al. (1998) J Biol Chem 273: 25552-25555. 3. Luo S., et al (2006) 26 (15): 5688-97. 4. Yu Z., et al. (1999) Exp Neurol. 15: 302-314. 5. Koomagi R., et al. (1999) Anticancer Res. 19:4333-4336. 6. Laquerre S., et al. (1998) J. Virology 72: 4940-4949. 7. Dong D., et al. (2005) Cancer Res 65(13): 5785-91.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Specificity

Detects ~78kDa.

Background Reference 01

1. Cho S., et al. (2007) Mol Cell Biol. 27 (1) : 368-83. 2. Yang Y., et al. (1998) J Biol Chem 273: 25552-25555. 3. Luo S., et al (2006) 26 (15) : 5688-97. 4. Yu Z., et al. (1999) Exp Neurol. 15: 302-314. 5. Koomagi R., et al. (1999) Anticancer Res. 19:4333-4336. 6. Laquerre S., et al. (1998) J. Virology 72: 4940-4949. 7. Dong D., et al. (2005) Cancer Res 65 (13) : 5785-91.