DUX4 Antibody, Clone P2B1

Mouse Anti-Human DUX4 Monoclonal IgG1

Product Specifications

Background

DUX4 (Double Homeobox 4) is a transcription factor implicated in gene regulation during early development. In adult tissues, aberrant expression of DUX4 is associated with toxic gain-of-function effects, particularly in facioscapulohumeral muscular dystrophy (FSHD) . Recent studies suggest that DUX4 may also contribute to neurodegenerative processes by activating stress response genes, disrupting RNA metabolism, and inducing apoptosis. Its expression in differentiated tissues, including skeletal muscle and potentially the nervous system, highlights its pathological relevance beyond muscular dystrophy. DUX4’s ability to modulate transcriptional networks and trigger cell death pathways positions it as a candidate for further investigation in neurodegeneration, especially in disorders involving epigenetic dysregulation and inflammatory signaling.

CAS Number

9007-83-4

Specifications

Detects ~45kDa. No cross-reactivity with DUX4c.

Product Name Alternative

Double homeobox 4, Double homeobox 10, DUX10, Double homeobox protein 4/10, DUX4L, DUX4L1

Synonyms

DUX4 Antibody, Clone P2B1

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

22947

Swiss Prot

F5GZ66

Accession Number

NP_149418.3

Cellular Locus

Nucleus

Host

Mouse

Species Reactivity

Human,Mouse

Immunogen

C-terminal 76 amino acids of Human DUX4 with glutathione-s-transferase (gst) tag

Target

DUX4

Clonality

Monoclonal

Isotype

IgG1

Clone

P2B1

Conjugation

Unconjugated

Type

Monoclonal

Applications

WB | IHC | ICC/IF

Validated Applications

WB,IHC,ICC/IF

Field of Research

Stem Cells | Mesenchymal Stem Cells | Myogenesis

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-192 was sufficient for detection of DUX4 in 20 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG: HRP as teh secondary.

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:1000) ; optimal dilutions for assays should be determined by the user.

Weight

0.012

Buffer

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Entrez Gene: “Dux4 Double Homeobox, 4” 2. Dixit M., et al., (2007) Proc. Natl Acad Sci. USA. 104(46): 18157-18162. 3. Kowaljow V., et al. (2007) Neuromuscl Disord. 17(8): 611-623. 4. Lemmers R., et al. (2010) Science Express. 329(5999): 1650-1653. 5. Snider L., et al. (2010) PLoS Genetics. 6:1-14.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

-20ºC

Specificity

Detects ~45kDa. No cross-reactivity with DUX4c.

Background Reference 01

1. Entrez Gene: “Dux4 Double Homeobox, 4” 2. Dixit M., et al., (2007) Proc. Natl Acad Sci. USA. 104 (46) : 18157-18162. 3. Kowaljow V., et al. (2007) Neuromuscl Disord. 17 (8) : 611-623. 4. Lemmers R., et al. (2010) Science Express. 329 (5999) : 1650-1653. 5. Snider L., et al. (2010) PLoS Genetics. 6:1-14.